Effects of Different Drying Methods on Content of Paederosidic Acid in Paederia scandens  

作　　者：Jingrong LU Haisheng ZENG Silu HE Xiumei MA Dongmei HUANG Guilin YANG Chengtong LIU Qiji ZHOU 

机构地区：[1]Guangxi International Zhuang Medicine Hospital, Nanning 530201, China [2]The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China

出　　处：《Medicinal Plant》2020年第6期59-61,66,共4页药用植物：英文版

基　　金：The First Batch High-level Talent Scientific Research Project of The Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311);Project for Improving Basic Research Ability of Young and Middle-aged and Young Teachers in Colleges and Universities of Guangxi in 2020(2020KY13034);Program of Youjiang Medical University for Nationalities(yy2018ky018).

摘　　要：[Objectives]To establish a method to simultaneously determine the content of paederosidic acid in Paederia scandens dried by four different methods.[Methods]Reversed-phase high performance liquid chromatography(RP-HPLC)was adopted.The chromatographic column:Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase:acetonitrile-0.1%phosphoric acid aqueous solution;gradient elution;column temperature:30℃;flow rate:1 mL/min;detection wavelength:236 nm;injection volume:10μL.[Results]The linear range of the detection injection volume of paederosidic acid was 0.64-9.60μg(R=0.9992);the limit of quantity(LOQ)was 5.10 ng,and the limit of detection(LOD)was 1.36 ng;the RSD of the precision,stability and reproducibility test was all less than 3%;the sample recovery rate was 98.87%,RSD<3.00,n=6.The results show that the content of paederosidic acid in the shade-dried P.scandens is the highest,and the order of the content is P.scandens(dry in shade)>P.scandens(dried at 50℃)>P.scandens(dried at 60℃)>P.scandens(dried at 70℃).[Conclusions]This method is sensitive,reliable and reproducible,and can be used to simultaneously determine the content of paederosidic acid in P.scandens dried by four different methods.

关 键 词：Paederia scandens(Lour)Mreeill Paederosidic acid Reversed-phase high performance liquid chromatography(RP-HPLC) Content determination 

分 类 号：R28[医药卫生—中药学]