Thin-layer Identification,Extraction Process and Content Determination of Chlorogenic Acid in Laggera alata(D.Don)Sch.Bip.ex Oliv.  

作　　者：Qiaofeng QIN Xue JIANG Jiangcun WEI Jiabao MA Jingrong LU Lifu WEI Xiumei MA Xuanyang ZENG 

机构地区：[1]The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China [2]Guangxi International Zhuang Medicine Hospital, Nanning 530201, China [3]Guangxi Zhuang Yao Medicine Center of Engineering and Technology, Nanning 530200, China

出　　处：《Medicinal Plant》2020年第6期72-76,共5页药用植物：英文版

基　　金：Basic Research Ability Enhancement Project for Young and Middle-aged Teachers in Colleges and Universities of Guangxi in 2019(No.2019KY0341);National Traditional Chinese Medicine Special Technology Inheritance Talent Training Project 2016;Open Project of Guangxi Zhuang Yao Pharmaceutical Engineering Technology Research Center(No.KJT1900105);Youth Foundation of Guangxi University of Chinese Medicine(No.2019QN036).

摘　　要：[Objectives]This paper aims to establish thin-layer identification and content determination method for Laggera alata(D.Don)Sch.Bip.ex Oliv.with chlorogenic acid as the index component and compare the content of chlorogenic acid in L.alata from different places in Guangxi.[Methods]Silica gel GF254 thin-layer plate was used for identification under an ultraviolet lamp(365 nm),with butyl acetate-formic acid-water(V∶V∶V=7∶2.5∶2.5)as a developing agent.The content of chlorogenic acid was determined under the following chromatographic conditions:column,Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase,methanol-0.1%phosphoric acid(28∶72);detection wavelength,329 nm;flow rate,1.0 mL/min;column temperature,25℃;and injection volume,10μL.[Results]Chlorogenic acid can be detected by thin layer chromatography with clear spot and good specificity.Chlorogenic acid showed a good linear relationship in the injection amount range of 0.099-0.99μg(R^(2)=0.9999).The content of chlorogenic acid in L.alata varied greatly among the 10 different producing areas in Guangxi.L.alata produced in Dee Township,Longlin,Baise,Guangxi showed the highest chlorogenic acid content,and that produced in Shangsi County and Pingle County showed the lowest chlorogenic acid content.[Conclusions]This method can effectively identify L.alata and accurately determine the content of chlorogenic acid,thereby providing a scientific basis for the development and utilization of L.alata resources.

关 键 词：Laggera alata Chlorogenic acid Content determination Thin-layer identification Extraction process 

分 类 号：R28[医药卫生—中药学]