基于二硫化钼纳米片类过氧化物酶活性比色法测定尿酸  

Colorimetric Determination of Uric Acid with Molybdenum Disulfide Nanosheets as Peroxidase Mimetic Enzyme

作　　者：石睿 覃秀 袁春玲 成诗琦 王益林[1] SHI Rui;QIN Xiu;YUAN Chun-Ling;CHENG Shi-Qi;WANG Yi-Lin(School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,南宁530004

出　　处：《分析化学》2021年第3期397-406,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.61664002);广西研究生教育创新计划项目(No.YCSW2020016)资助。

摘　　要：采用一步水热法制备具有类过氧化物酶活性的MoS_(2)纳米片,并基于MoS_(2)纳米片可催化H_(2)O_(2)氧化3,3′,5,5′-四甲基联苯胺(TMB)生成蓝色氧化产物(ox-TMB)的原理,建立了测定H_(2)O_(2)浓度的分析方法。在pH 3.7、温度35℃、MoS_(2)浓度1.0μg/mL及孵育时间40 min的条件下,ox-TMB的吸光度随H_(2)O_(2)浓度的升高而增大。基于H_(2)O_(2)是尿酸氧化酶催化尿酸氧化的产物之一,建立了MoS_(2)纳米片催化H_(2)O_(2)氧化TMB间接测定尿酸的分析方法。在2.5~40μmol/L和40~100μmol/L范围内,检测体系的吸光度与尿酸浓度呈良好的分段线性关系,方法在低浓度区间的检出限为0.093μmol/L,应用于血清和尿样中尿酸的测定,回收率为92.6%~102.0%。MoS_(2)nanosheets with peroxidase-like activity were prepared by one-step hydrothermal method.The obtained MoS_(2)nanosheets could catalyze H_(2)O_(2)-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine(TMB)to produce corresponding blue oxidized product(ox-TMB).Thus,a simple method for detection of H_(2)O_(2) was established.Under the optimized conditions such as pH 3.7,temperature of 35℃,1.0μg/mL MoS_(2)and incubation time of 40 min,the absorbance of ox-TMB increased with the increase of H_(2)O_(2) concentration.Given the fact that H_(2)O_(2) was one of the products of uric acid oxidase-catalyzed uric acid oxidation,a method for the indirect detection of uric acid based on MoS_(2)nanosheets-catalyzed and H_(2)O_(2)-mediated TMB oxidation was developed.The increase of ox-TMB absorbance enabled the sensitive determination of uric acid in the lineatrange of 2.5-40μmol/L and 40-100μmol/L,respectively,and the detection limit was 0.093μmol/L(3σ/k).The method exhibited potential application in the detection of uric acid in urine and serum samples with recoveries of 92.6%-102.0%.

关 键 词：二硫化钼 纳米片 模拟酶 检测 尿酸 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]