夹心式碳纳米管/金复合材料电化学适配体传感器的构建及其对双酚A的检测性能  

Construction of Electrochemical Aptasensor Based on SandwichCarbon Nanotube/Gold Film for Detection of Bisphenol A

作　　者：李海玉[1] 李文雅[1] 王婉[1] 吕庆[1] 王志娟[1] 张庆[1] LI Hai-Yu;LI Wen-Ya;WANG Wan;LYU Qing;WANG Zhi-Juan;ZHANG Qing(Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176

出　　处：《分析化学》2021年第3期415-423,共9页Chinese Journal of Analytical Chemistry

基　　金：国家重点研发计划(No.2016YFF0203703);市场监管业务综合保障经费-工业及消费品质量安全保障项目资助。

摘　　要：研制了一种用于检测双酚A的电化学适配体传感器。在金膜工作电极表面修饰溅射镀金的多壁碳纳米管,形成夹心式结构,能够有效提高电极表面积和电子传输。3,3′,5,5′-四甲基联苯胺(TMB)作为染料信号分子结合到固定于电极表面的双酚A适配体中,形成复合物(ssDNA-TMB)。当双酚A存在时,其与适配体特异性结合,引起适配体构象发生变化,ssDNA-TMB中的TMB分子被释放出来,导致电极表面的TMB电化学信号强度降低。采用差分脉冲伏安法进行测试,在0.05~500 nmol/L浓度范围内,双酚A的浓度与TMB的电化学信号呈线性关系,检出限为43 pmol/L。实际塑料样品中双酚A的加标回收率在88.9%~110.2%之间,相对标准偏差在2.7%~9.0%之间。本方法简便高效,适用于现场检测,在消费品安全监管中具有良好的应用前景。An electrochemical aptasensor for detection of bisphenol A(BPA)was developed in this work.Disposable gold-film working electrode was modified with gold-coated multi-walled carbon nanotubes to form a sandwich structure,which could greatly enhance the surface area of the working electrode and electronic transport.TMB(3,3′,5,5′-tetramethylbenzidine),acting as signal molecule,was intercalated into the immobilized aptamer to form a ssDNA-TMB complex.In the presence of BPA,the immobilized aptamer would have a conformational change,leading the intercalated TMB to be released from the complex.As a result,the electrochemical signal of the intercalated TMB was decreased.The change of peak current measured using differential pulse voltammetry was linearly correlated with the natural logarithm of concentration of BPA from 0.05 nmol/L to 500 nmol/L.The limit of detection was 43 pmol/L.The recoveries in real samples at different spiked levels were in the range of 88.9%-110.2%,and the relative standard deviations(RSDs)were 2.7%-9.0%.The proposed aptasensor here was simple,efficient and suitable for field testing,showing good potential for the supervision of consumer products safety.

关 键 词：碳纳米管复合材料 3 3′ 5 5′-四甲基联苯胺 适配体传感器 双酚A 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O657.1[自动化与计算机技术—控制科学与工程]