10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究  

Investigation ofα-Amylase and Tyrosinase Inhibitory Activities,Phenolic Compounds,and Antioxidant Activity in Ten Kinds of Wild Fruits

作　　者：田丝竹 李绪文[1] 臧爽 张子微 张寒琦[1] 于永[2] TIAN Si-Zhu;LI Xu-Wen;ZANG Shuang;ZHANG Zi-Wei;ZHANG Han-Qi;YU Yong(College of Chemistry,Jilin University,Changchun 130012,China;College of Instrumentation and Electrical Engineering,Jilin University,Changchun 130061,China)

机构地区：[1]吉林大学化学学院,长春130012 [2]吉林大学仪器与电气工程学院,长春130061

出　　处：《分析化学》2021年第3期449-459,共11页Chinese Journal of Analytical Chemistry

基　　金：吉林省教育厅“十三五”科学技术研究项目(No.2016-409);吉林省科技发展计划项目(Nos.20180201059SF,20180201027GX)资助。

摘　　要：考察了10种可食用野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用,结果表明,山荆子果实提取物对α-淀粉酶的抑制能力最强,其IC_(50)值与常见的α-淀粉酶抑制剂阿卡波糖接近。因此,山荆子果提实取物有望用于治疗非胰岛素依赖型糖尿病。采用1,1-二苯基苦基苯肼(DPPH)法测定了水果的抗氧化活性,采用Folin-Ciocalteu比色法和pH示差法测定总酚含量和总花青素含量,采用高效液相色谱(HPLC)法测定7种酚酸的含量。结果表明,10种野生水果对酪氨酸酶的抑制能力与其总酚含量和抗氧化活性均高度相关。冷冻野生水果中总酚的含量范围为617.3~7447.4μg/g,其中,8种野生水果显示出高抗氧化能力。新鲜水果和冷冻水果的抗氧化能力之间的显著相关性表明,在液氮中快速冷冻水果并磨粉的保存方法对野生水果的抗氧化活性影响较小。Ten kinds of edible wild fruits from China,especially northeast China,were analyzed for their α-amylase and tyrosinase inhibitory activities.The extract of the fruit of dwarf apple had the highestα-amylase inhibitory activities and its IC_(50)value was close to that of acarbose,which indicated that the extract of the fruit of dwarf apple could be a good candidate for the treatment of non-insulin dependent diabetes.The antioxidant capacity was determined using DPPH method.The total phenolics and total anthocyanins were determined by Folin-Ciocalteu method and pH-differential method,respectively.The contents of seven phenolic acids were determined by high-performance liquid chromatography(HPLC).The tyrosinase inhibitory activity was found to be highly correlated with both total phenolic content and antioxidant capacity.The contents of total phenolics in the frozen wild fruits ranged from 617.3 to 7447.4μg/g.Eight out of ten wild fruits showed high antioxidant capacity.A significant correlation between the antioxidant capacity of raw(fresh)and frozen fruits indicated that fast freezing fruit powder in liquid nitrogen followed by fast thawing did not damage the antioxidant properties of the wild fruits.

关 键 词：野生水果 α-淀粉酶抑制活性 酪氨酸酶抑制活性 酚酸 高效液相色谱 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]