A mediator-free self-powered glucose biosensor based on a hybrid glucose/MnO_(2) enzymatic biofuel cell  

作　　者：Shuai Hao He Zhang Xiaoxuan Sun Junfeng Zhai Shaojun Dong 

机构地区：[1]State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,130022,China [2]Department of Chemistry,University of Science and Technology of China,Hefei,230026,China

出　　处：《Nano Research》2021年第3期707-714,共8页纳米研究（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.21721003 and 21675151);the Ministry of Science and Technology of China(No.2016YFA0203203).

摘　　要：Self-powered glucose biosensor(SPGB)is of great interest due to the advantages including single configuration,good stability and particularly no need of external power sources.Herein,a mediator-free SPGB with high sensitivity and good selectivity is constructed based on a hybrid enzymatic biofuel cell(EBFC)composed of a glucose oxidase/cobalt phthalocyanine/1-pyrenebutyric acid/buckypaper(GOD/CoPc/PBA/BP)bioanode and a MnO_(2)/PBA/BP capacitive cathode.The efficient electron transfer from GOD to electrodes is achieved successfully through the anode oxidation of hydrogen peroxide(H_(2)O_(2)),one nature product of glucose oxidation catalyzed by GOD,thus avoiding the potential drawbacks posed by the use of redox mediators.CoPc servers as an efficient catalyst to lower the anode potential required by the reaction of H_(2)O_(2) to 0.17 V.The MnO_(2)/PBA/BP capacitive cathode is utilized because it can not only provide a high discharge potential and adequate capacitance to match the bioanode well,but also exhibit no potential interference to the anodic reaction.The concentration of glucose can be detected simply by measuring the output of the SPGB and a wide linear detection range from 0.5 to 8 mM has been obtained with high sensitivities of 48.66 and 32.12μA·cm^(−2)·mM^(−1) with and without stirring,respectively.The recoveries of glucose in grape juice and human serum are in the range from 99.5%to 101.2%with the relative standard deviation(RSD)less than 8%,indicating the good promise of the SPGB in sensing glucose in real samples.

关 键 词：self-powered glucose biosensor glucose oxidase mediator-free hydrogen peroxide capacitive cathode 

分 类 号：TM911[电气工程—电力电子与电力传动] TP212[自动化与计算机技术—检测技术与自动化装置]