Molecular Characterization and Expression Analysis of SKIV Infection of Interferon-Induced Protein with Tetratricopeptide Repeats 1(IFIT1) in Epinephelus lanceolatus  

作　　者：WANG Lei MA Teng XU Wenteng CHEN Zhangfan ZHOU Qian ZHENG Guiliang CHEN Songlin 

机构地区：[1]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences(CAFS),Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China [2]College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,China [3]College of Marine Life Sciences,Ocean University of China,Qingdao 266237,China

出　　处：《Journal of Ocean University of China》2021年第2期383-392,共10页中国海洋大学学报（英文版）

基　　金：supported by the Shandong Breeding Project (No. 2016LZGC009);the Projects from Laboratory for Marine Fisheries Science and Food Production Processes;Pilot National Laboratory for Marine Science and Technology (Qingdao)(Nos. 2018-MFS-T08, 2017A STCP-OS15);the Central Public-interest Scientific Institution Basal Research Fund,CAFS (No. 2020TD20);the Central Public-Interest Scientific Institution Basal Re-search Fund,YSFRI,CAFS (No. 20603022018026)。

摘　　要：Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.

关 键 词：interferon-induced protein with tetratricopeptide repeats 1(IFIT1) Epinephelus lanceolatus tetratricopeptide repeats(TPR)motif expression pattern antiviral function 

分 类 号：S941.4[农业科学—水产养殖]