二倍体与四倍体刺槐组培苗形态和生理性状比较  

Comparison of Morphological and Physiological Characteristics of Tissue Culture Plantlets of Diploid and Tetraploid Robinia pseudoacacia

作　　者：李秀宇 董黎[1] 孙宇涵[1] 李健康 刘晓迪 谭红岩 Saleem Uddin 李云[1] LI Xiuyu;DONG Li;SUN Yuhan;LI Jiankang;LIU Xiaodi;TAN Hongyan;SALEEM Uddin;LI Yun(Beijing Advanced Innovation Center for Tree Breeding by Molecular Design/Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration/National Engineering Laboratory for Tree Breeding/College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;State-owned Huangtuliangzi Forest Farm,Pingquan,Hebei 067500)

机构地区：[1]北京林木分子设计育种高精尖创新中心/国家林业与草原局刺槐工程技术研究中心/林木育种国家工程实验室/北京林业大学生物科学与技术学院,北京100083 [2]河北省平泉市国有黄土梁子林场,河北平泉067500

出　　处：《核农学报》2021年第3期605-612,共8页Journal of Nuclear Agricultural Sciences

基　　金：北京林业大学重大科研成果培育项目(2017CGP007);国家自然科学基金项目(31570677);国家重点研发课题(2017YFD0600503);国家林业和草原局科技发展中心项目(2016007)。

摘　　要：为了比较二倍体与四倍体刺槐组培苗不同无性系之间的差异,本研究分别测定二倍体刺槐组培苗和人工诱导四倍体刺槐组培苗的形态及生理指标,并进行相关分析。结果表明,多数四倍体刺槐无性系的长势、抗逆性优于二倍体;二倍体与四倍体刺槐不同无性系以及不同形态、生理指标之间存在丰富的变异,其中四倍体刺槐的变异更为丰富;各测定指标之间存在较为复杂的相关关系,在选择育种时,需综合考虑。根据试验结果,结合育种目标,可选育出优良四倍体刺槐无性系,丰富刺槐种质资源,增加遗传多样性。本研究为刺槐倍性育种工作提供了理论依据和科学参考。In order to compare the differences of different clones of diploid and tetraploid Robinia pseudoacacia tissue culture plantlets, the morphological and physiological indexes of the diploid and artificially induced tetraploid tissue culture plantlets of Robinia pseudoacacia were determined and analyzed. The results showed that the growth and stress resistance of most tetraploid Robinia pseudoacacia clones were better than that of diploid clones;there were abundant variations between and with diploid and tetraploid Robinia pseudoacacia clones in different morphological and physiological indices, but the variation of tetraploid Robinia pseudoacacia was more abundant;there were complex correlations among the tested indices. Comprehensive consideration should be taken in selection and breeding. According to the results of the experiment and the breeding objectives, the excellent tetraploid Robinia pseudoacacia clones can be selected to enrich the germplasm resources of Robinia pseudoacacia and increase its genetic diversity.

关 键 词：刺槐 二倍体 四倍体 形态指标 生理指标 

分 类 号：S792.27[农业科学—林木遗传育种]