青海高原油菜蜜稳定同位素产地特征与环境响应机制研究  

Stable Isotope Signatures of Rape Honey in Qinghai Province and Their Response Mechanisms to Environmental Factors

作　　者：谭阳 刘志 韩燕[1] 崔明明[1] 肖明[1] 耿贵工[1] 孙小凤[1] 袁玉伟[2] TAN Yang;LIU Zhi;HAN Yan;CUI Mingming;XIAO Ming;GENG Guigong;SUN Xiaofeng;YUAN Yuwei(Academy of Agriculture and Forestry Sciences,Qinghai University/Laboratory of Quality&Safety Risk Assessment for Agro-products,Ministry of Agriculture and Rural Affairs,Xining,Qinghai 810016;Institute of Quality and Standard for Agro-product,Zhejiang Academy of Agricultural Sciences/Key Laboratory of Information Traceability of Agricultural Products,Ministry of Agriculture and Rural Affairs,Hangzhou,Zhejiang 310021)

机构地区：[1]青海大学农林科学院/农业农村部农产品质量安全风险评估实验室,青海西宁810016 [2]浙江省农业科学院农产品质量标准研究所/农业农村部农产品信息溯源重点实验室,浙江杭州310021

出　　处：《核农学报》2021年第3期635-642,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家农产品质量安全风险评估重大专项(GJFP201701004)。

摘　　要：为探究青海油菜蜜稳定同位素特征及影响因素,本研究利用元素分析-稳定同位素比率质谱(EA-IRMS)建立了油菜蜜总δ^(13)C值及其内源蛋白质δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值的检测方法,分析了青海不同产地、不同年份油菜蜜及其内源蛋白质同位素特征差异,探讨了油菜蜜内源蛋白质稳定同位素与产地环境之间的关系。结果表明,青海省区域内油菜蜜内源蛋白质的δ^(13)C值和δ^(15)N值较稳定,不同产地、不同年份间无显著差异;不同年份油菜蜜内源蛋白质的δ^(18)O值差异极显著(P<0.01),不同产地油菜蜜内源蛋白质的δ^(18)O值及δ2H值均存在极显著差异(P<0.01)。在环境影响因素中,油菜蜜内源蛋白质δ^(18)O值与生产季节降水量呈显著负相关(r=-0.822,P<0.05)。本研究结果为青海高原油菜蜜真实性鉴别与产地溯源研究提供了理论依据与技术支撑。In order to explore the characteristics and influencing factors on stable isotopes of Qinghai rape honey, a detection method using elemental analysis-stability isotope ratio mass spectrometry(EA-IRMS) was established to obtain the total δ^(13)C value of rape honey and its δ^(13)C, δ^(15)N, δ^(2)H, δ^(18)O values of endogenous proteins. The stable isotope variations of rape honey and its endogenous proteins were analyzed across different years and producing areas in Qinghai. The relationship between stable isotope of endogenous protein in rape honey endogenous protein and the origin environment was also discussed. The results showed that the δ13C and δ15N values of endogenous proteins in Qinghai rape honey were considerably stable, and there were no significant differences among different years and different origins. The δ^(18)O values of inter-annual in endogenous proteins of rape honey samples were significantly different, as well as δ^(18)O and δ^(2)H values of endogenous proteins from different regions. For the environment factors, δ^(18)O value in endogenous proteins of rape honey was negatively correlated with precipitation during the production season(r=-0.822). The results of this study provided theoretical basis and technical support for the authenticity identification and origin traceability of Qinghai rape honey.

关 键 词：油菜蜜 内源蛋白质 稳定同位素分析 产地环境因子 分馏响应机制 

分 类 号：S896.1[农业科学—特种经济动物饲养]