水稻粒形与千粒质量的QTL分析  

QTL analysis of rice grain shape and thousand-grain weight

作　　者：李金吉 张银霞[1,2] 赵娜 田蕾[1,2] 杨淑琴[1,2] 李培富 LI Jinji;ZHANG Yinxia;ZHAO Na;TIAN Lei;YANG Shuqin;LI Peifu(College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan,Ningxia 750021,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏优势特色作物现代分子育种重点实验室,宁夏银川750021

出　　处：《西北农林科技大学学报（自然科学版）》2021年第2期54-60,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：宁夏农业育种专项水稻新品种选育项目(2018NYYZ0302)。

摘　　要：【目的】挖掘控制水稻粒形和千粒质量的QTL位点,为水稻高产育种提供有利资源。【方法】以水稻大粒品种大粒稻和小粒品种东北小粒种杂交获得的256个单株F 2群体为材料,利用124对亲本间多态性SSR标记对控制粒长、粒宽、长宽比和千粒质量等性状进行QTL分析。【结果】F 2群体粒形性状均呈现连续变异的双峰分布,且粒长、长宽比和千粒质量分布均偏向于大粒亲本。相关分析表明,粒长、粒宽和千粒质量性状间均呈极显著正相关。共检测到17个QTL位点,与粒长、粒宽、长宽比和千粒质量有关的QTL位点分别为6,3,5和3个,分布于水稻第2、3、4、5、9、10号染色体上,贡献率为4%~18%。贡献率大于10%的主效QTL位点有4个,分别为qGL-2-1、qGW-2-2、qGW-2-3和qL/W-2-1,其中qGW-2-2、qGW-2-3和qL/W-2-1与克隆的粒宽基因GW2在同一区间内,可能为同一基因,qGL-2-1可能是新的控制粒形的主效QTL位点;其余贡献率小于10%的位点也可能有新位点。【结论】检测到的QTLs具有稳定性,可用于控制水稻粒形性状表现的基因精细定位及克隆。【Objective】This study identified the QTL loci for controlling rice grain size and thousand-grain weight to provide favorable resources for high-yield rice breeding.【Method】The rice F 2 population consisting of 256 individual plants derived from a cross was obtained using large-grain varieties and small-grain varieties of Northeast small-grain varieties as materials.A total of 124 SSR markers were used to perform QTL mapping analysis for controlling grain length,grain width,length-width ratio and thousand grain weight.【Result】The F 2 population showed a bimodal distribution of continuous variation in grain shape traits.The distributions of grain length,aspect ratio and thousand grain weight were all biased towards large-grain parents.The correlation analysis showed that there was a significant positive correlation between grain length,grain width and thousand-grain weight traits.A total of 17 QTLs were detected,and the QTLs related to grain length,grain width,aspect ratio,and thousand-grain weight were 6,3,5,and 3,respectively.They were mainly distributed on chromosomes 2,3,4,5,9,and 10 with contribution rate of 4%to 18%.There were 4 main QTLs of qGL-2-1,qGW-2-2,qGW-2-3 and qL/W-2-1 with a phenotypic contribution rate greater than 10%.Among them,qGW-2-2,qGW-2-3 and qL/W-2-1 were in the same interval as the cloned gene GW2 with the possibility of being the same gene,while qGL-2-1 might be a new gene controlling grain type.The remaining sites with contribution rate of less than 10%could also have new sites.【Conclusion】The detected QTLs with stability can provide basis for fine mapping and cloning of genes controlling rice grain shape traits.

关 键 词：水稻 粒形 遗传图谱 QTL定位 水稻育种 

分 类 号：S511.033[农业科学—作物学]