酶联免疫吸附法测定家禽配合饲料中氯霉素含量结果探析  

Analysis on the Determination of Chloramphenicol in Poultry Compound Feed by Enzyme-linked Immunosorbent Assay

作　　者：刘宏梅 韦文飞 农爱红 卢少坤 陆必科 Liu Hong-mei;Wei Wen-fei;Nong Ai-hong;Lu Shao-kun;Lu Bi-ke(Baise Animal Disease Prevention and Control Center,Baise,Guangxi 533000,China)

机构地区：[1]百色市动物疫病预防控制中心,广西百色市533000

出　　处：《广西农学报》2020年第5期26-28,共3页Journal of Guangxi Agriculture

摘　　要：为验证酶联免疫吸附法(ELISA)对家禽配合饲料中氯霉素(CAP)含量检测的实用性,根据氯霉素残留酶联免疫检测试剂盒操作说明对样本进行前处理,以0.1μg/kg、0.3μg/kg、0.5μg/kg浓度的氯霉素标准溶液进行空白添加回收实验,结果回收率在70%~120%以内,变异系数在5%以下。实验结果表明所使用的ELISA试剂盒能够满足检测饲料中氯霉素残留的需要,适合用于基层工作中大规模筛查氯霉素残留量。In order to verify the practicability of enzyme-linked immunosorbent assay(ELISA)in the de⁃tection of chloramphenicol(CAP)in poultry compound feed,the samples were pretreated according to the operating instructions of chloramphenicol residue enzyme-linked immunosorbent assay kit.The recovery rate was less than 70%~120%and the coefficient of variation was less than 5%with 0.1μg/kg、0.3μg/kg、0.5μg/kg chloramphenicol standard solution.Experimental results show that the ELISA kit can meet the needs of detection of chloramphenicol residues in compound feed and is suitable for large-scale screening of chloramphenicol residues in grass-roots work.

关 键 词：饲料 氯霉素 酶联免疫吸附法 

分 类 号：S859.84[农业科学—临床兽医学]