霸王花不同部位粗多糖的结构组成及体外抗氧化活性  

Structual Composition and in Vitro Antioxidant Activities of Crude Polysaccharide from Different Parts of Flower of Hylocereus Undatus

作　　者：冯峰[1,2] 杨淑玲[3] 张奇 吴岩斌 FENG Feng;YANG Shuling;ZHANG Qi;WU Yanbin(Department of Pharmacy,Fujian Provincial Governmental Hospital,Affiliated Hospital of Fujian Health College,Fuzhou 350003,China;Department of Pharmacy,Fujian Health College,Fuzhou 350101,China;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建省级机关医院,福建卫生职业技术学院附属医院药剂科,福州350003 [2]福建卫生职业技术学院药学系,福州350101 [3]福建中医药大学药学院,福州350122

出　　处：《中国现代应用药学》2021年第2期189-195,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：福建省中青年教师教育科研项目(JAT171042)。

摘　　要：目的研究霸王花不同部位粗多糖的结构组成和体外抗氧化活性。方法利用水提醇沉法制备霸王花4个不同部位(花瓣、花萼、雌蕊、雄蕊)的粗多糖,通过苯酚-硫酸法、考马斯亮蓝法、凝胶排阻色谱联用多角度激光光散射和示差折光仪法[size exclusion chromatography(SEC) coupled with multiangle laser light scattering(MALLS)and refractive index detector(RID),SEC-MALLS-RID]、高效液相色谱法、傅里叶红外光谱法(Fourier transform infrared spectrometer,FTIR)分析比较霸王花不同部位粗多糖结构特征;以对2,2-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐自由基、羟基自由基和超氧阴离子自由基的清除作用为指标,评价霸王花不同部位粗多糖的体外抗氧化活性。结果霸王花不同部位粗多糖中多糖含量为52.20%~61.52%,蛋白质含量为1.17%~3.21%;霸王花不同部位粗多糖均含有D-甘露糖、L-鼠李糖、D-葡萄糖醛酸、D-半乳糖醛酸、葡萄糖、D-半乳糖、D-木糖、D-阿拉伯糖,但单糖的比例有差异;4个不同部位粗多糖的SEC色谱图、重均分子质量及其分布、FTIR色谱图具有相似性;霸王花不同部位粗多糖具有体外抗氧化活性。结论霸王花不同部位粗多糖结构组成具有较高的相似性,均具有良好的抗氧化活性,在保健食品和药品等领域具有良好的开发利用前景。OBJECTIVE To investigate the structual composition and in vitro antioxidant activity of crude polysaccharide from different parts of flower of Hylocereus undatus. METHODS The crude polysaccharide from petals, calyx, pistil and stamens in 4 different parts of flower of Hylocereus undatus were extracted by method of water extraction and alcohol precipitation. Structural characteristics of crude polysaccharide from different parts of flower of Hylocereus undatus were investigated and compared using phenol-sulfuric acid method, coomassie brilliant blue method, size exclusion chromatography(SEC) coupled with multiangle laser light scattering and(MALLS) refractive index detector(RID)(SEC-MALLS-RID), HPLC and Fourier transform infrared spectrometer(FTIR), respectively. The 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate acid) radical, hydroxyl radical and superoxide anion radical scavenging activities was assayed to evaluate the antioxidant activity in vitro of crude polysaccharide from different parts of flower of Hylocereus undatus. RESULTS The results demonstrated that the contents of polysaccharides were 52.20%-61.52%, the protein contents were 1.17%-3.21% in crude polysaccharide of different parts from Hylocereus undatus. All polysaccharides contained D-mannose, L-rhamnose, D-glucuronic acid, D-galacononic acid, glucose, D-galactose, D-xylose and D-arabinose, but the proportion of monosaccharides varied remarkably in the crude polysaccharides from different parts of flower of Hylocereus undatus. The SEC chromatograms, the molecular weights and their distribution, and the FTIR chromatograms of crude polysaccharides from different parts of flower of Hylocereus undatus were similar. The crude polysaccharide from different parts of flower of Hylocereus undatus had significant anti-oxidative activities in vitro. CONCLUSION The structual composition of crude polysaccharide from different parts of flower of Hylocereus undatus have high similarity and significant anti-oxidative activities in vitro, and can be a resourc

关 键 词：霸王花 多糖 单糖组成 凝胶排阻色谱联用多角度激光光散射和示差折光仪法 红外光谱分析法 

分 类 号：R284.1[医药卫生—中药学]