2个北美冬青新品种组培快繁高效再生技术体系的建立  

Establishing an Efficient Regeneration Protocol in vitro for Rapid Micro-propagation of Two New Varieties of Ilex verticillata L.

作　　者：刘瑞[1,2] 张嘉仪 葛超奇 谢露露 陈颖 张粲[1,2] 陈茜 LIU Rui;ZHANG Jia-Yi;GE Chao-Qi;XIE Lu-Lu;CHEN Ying;ZHANG Can;CHEN Xi(Collaborative Innovation Center of the Southern Modern Forestry,Nanjing 210037;College of Biology and the Environment,Nanjing Forest-ry University,Nanjing 210037;College of 2011,Nanjing Tech University,Nanjing 211816)

机构地区：[1]南京林业大学南方现代林业协同创新中心,南京210037 [2]生物与环境学院,南京210037 [3]南京工业大学2011学院,南京211816

出　　处：《植物研究》2021年第2期221-231,共11页Bulletin of Botanical Research

基　　金：江苏省科技厅现代农业重点项目(BE2017375);国家林业局948引进先进技术项目(2014-4-61);江苏省高校优势学科建设工程项目(PAPD);南京林业大学大学生创新训练计划项目(2019NFUSPITP0448)。

摘　　要：以北美冬青(Ilex verticillata L.)“Red Sprite”和“Winter Gold”2个品种的幼嫩枝条为外植体,建立了2个新品种的高效组培再生技术体系,为北美冬青新品种苗木的推广提供技术支持。结果表明:腋芽诱导的最佳培养基为MS+1.0 mg·L^(-1)6-苄氨基腺嘌呤(6-BA)+0.2 mg·L^(-1)萘乙酸(NAA),2个品种的诱导率都达到100%;最佳丛生芽增殖培养基为MS+0.1mg·L^(-1) NAA+0.5mg·L^(-1)6-BA+0.5 mg·L^(-1)氯吡苯脲(CPPU),其中品种“Red Sprite”的增殖系数达5.7,“Winter Gold”的增殖系数为4.5;最佳壮苗培养基为“Red Sprite”为MS+0.1 mg·L^(-1)NAA+0.5 mg·L^(-1)6-BA+1.0 mg·L^(-1)噻苯隆(thifenolone,TDZ)+1.0 mg·L^(-1)赤霉素(GA),培养25天时苗高达6.8 cm,“Winter Gold”的为MS+0.1 mg·L^(-1) NAA+0.5mg·L^(-1)6-BA+0.05mg·L^(-1) TDZ+1.0 mg·L^(-1)GA,25天时苗高达4.4 cm;最佳生根诱导培养基为1/2MS+0.4 mg·L^(-1) NAA+0.4 mg·L^(-1)吲哚丁酸(IBA)+1.0 g·L^(-1)活性炭(AC),“Red Sprite”品种生根率达95.0%,“Winter Gold”的生根率达97.0%;最佳移栽基质为泥炭土∶珍珠岩=3∶2,“Red Sprite”成活率为95.7%,“Winter Gold”的96.8%。An efficient and rapid micro-propagation system using tissue culture for two new varieties“Red Sprite”and“Winter Gold”of Ilex verticillata L.was established with the young shoots as explants,and an optimum method produced large numbers of genetically uniform seedlings and maintained its excellent character for I.verticillata L was offered.The optimal medium for axillary bud induction was MS+1.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) NAA,and the induction rate of both varieties were 100%respectively.The preferred medium for cluster bud proliferation was MS+0.1 mg·L^(-1) NAA+0.5 mg·L^(-1)6-BA+0.5 mg·L^(-1) CPPU.The highest proliferation coefficient was 5.7 for“Red Sprite”,and 4.5 for“Winter Gold”for 25 d culturing respectively.The optimal seedling medium for“Red Sprite”was MS+0.1 mg·L^(-1) NAA+0.5 mg·L^(-1)6-BA+1.0 mg·L^(-1) TDZ+1.0 mg·L^(-1) GA,and that of“Winter Gold”was MS+0.1 mg·L^(-1) NAA+0.5 mg·L^(-1)6-BA+0.05 mg·L^(-1) TDZ+1.0 mg·L^(-1) GA,the seedling height was up to 6.80 and 4.38 cm respectively.The optimal rooting induction medium was 1/2MS+0.4 mg·L^(-1) NAA+0.4 mg·L^(-1) IBA+1.0 mg•L^(-1) activated carbon,the rooting rate of“Red Sprite”and“Winter Gold”were 95.0%,or 97.0%respectively.The best transplanting substrates were peat∶perlite=3∶2,with a survival rate of 95.7%for“Red Sprite”and 96.8%for“Winter Gold”respectively.

关 键 词：北美冬青 品种“Red Sprite” 品种“Winter Gold” 组织培养 高效再生体系 

分 类 号：S68[农业科学—观赏园艺]