基于芽苗砧嫁接油茶砧穗创伤后内源激素动态变化分析  

Dynamic Changes of Endogenous Hormones in Rootstocks and Scions within Nurse Seedling Graft in Camellia oleifera Under Wound

作　　者：龙伟[1,2] 姚小华 吕乐燕[3] LONG Wei;YAO Xiao-Hua;LÜ Le-Yan(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400;Zhejiang Provincial Key Laboratory of Tree Breeding,Hangzhou 311400;Department of Hydraulic,Zhejiang Tongji Vocational College of Science and Technology,Hangzhou 311231)

机构地区：[1]中国林业科学研究院亚热带林业研究所,杭州311400 [2]浙江省林木育种技术研究重点实验室,杭州311400 [3]浙江同济科技职业学院,杭州311231

出　　处：《植物研究》2021年第2期232-242,共11页Bulletin of Botanical Research

基　　金：国家自然科学青年基金(31600504)。

摘　　要：为研究油茶(Camellia oleifera A)嫁接时穗条和砧木创伤后内源激素动态变化规律,解析影响砧穗嫁接面愈合的生理机制,为油茶砧穗愈合生长机理提供理论支持。以树龄6年的长林18号和53号的穗条和实生砧木为材料,按照芽苗砧嫁接方法切割穗条S0(0 min)、S10(10 min)、S40(40 min)和砧木茎段Z0(0 min)、Z10(10 min)后,利用液质联用法(HPLC-MS)测定吲哚乙酸(IAA)、脱落酸(ABA)、反式玉米素(TZR)、玉米素(Zeatin)、水杨酸(SA)和茉莉酸(JA)含量,分析不同时间段内源激素变化及品种间差异的关系。结果显示:创伤后18号的TZR、Zeatin和SA含量总体高于53号;18号IAA、SA和JA逐渐下降;TZR和Zeatin分别在S10和S0达最高值后下降;ABA在S10达最高值。53号IAA和JA爱S10达最高值后下降;TZR、Zeatin和SA在S10达低值后逐渐上升;ABA在S0达高值后逐渐下降。砧木茎段创伤前后激素含量除JA外18号高于53号;两品种Z0时激素含量下降,Z10后上升,仅53号ABA和SA含量在Z0达高值后下降。砧木茎段和根部激素含量在品种间除JA外18号高于53号,茎段的IAA、ABA高于根部,其他激素为根部高于茎段。激素比值在品种间和部位间差异明显;IAA/ABA、IAA/TZR、IAA/Zeatin和IAA/JA、ABA/TZR、ABA/Zeatin和ABA/JA比值为53号高于18号;穗条内SA/IAA为18号高于53号,SA/JA和SA/ABA为53号高于18号;砧木茎段均为18号高于53号;TZR/SA、TZR/JA比值在穗条和砧木茎段为18号高于53号。两品种创伤后IAA与JA极显著正相关,而IAA与SA,SA与JA在18号极显著正相关,53号极显著或显著负相关;53号TZR、Zeatin、SA间极显著或显著正相关,JA与TZR、Zeatin和SA极显著负相关。砧木茎段创伤后18号激素间为极显著或显著正相关;53号TZR和Zeatin与IAA、JA极显著正相关,与SA存在显著负相关,SA与JA有显著负相关。砧木茎段和根部间品种间仅在SA与各激素间相关性存在差异,其他激素间存在极显著正相关或负相关。综上�In order to analyze the physiological response to the surface healing of rootstocks and scions,and to provide the theoretical support for the growth mechanism,the dynamic changes of endogenous hormones in posttraumatic stress response in Camellia oleifera A.under wound were studied.The six-year-old Changlin 18 and Changlin 53 trees were used,and the contents of IAA,ABA,TZR,Zeatin,SA,JA were assayed using HPLCMS at different wound stages,and the changes of endogenous hormone in different period and relationship between varieties were analyzed.The results showed that the content of IAA,SA and JA decreased with the increasing time of wound in scions of Changlin 18,and the content of TZR and Zeatin increased first and decreased later which they were respectively reached the highest in S10 and S0.The content of ABA rose continually and reached the peak in S10.In scions of Changlin 53,the contents of IAA and JA would decrease after peak;the TZR,Zeatin,SA were gradually increased after minimum.The content of ABA was gradually decreased in S0 after maximum.The contents of endogenous hormones were higher in Changlin18 than in Changlin53 in stem of rootstock between varieties.The endogenous hormones were decreased in Z0 and increased in Z10 in varieties except the contents of ABA and SA in Changlin53 increased first and then decreased.In stem and root of rootstocks,the contents of endogenous hormones were higher in Changlin 18 than in Changlin 53 except JA;The contents of IAA and ABA in stem of rootstocks were higher than roots which other endogenous hormones in root of rootstocks were higher than in stem.There was difference in the ratio of hormone in varieties and part of rootstocks.The ratios of IAA/ABA,IAA/TZR,IAA/Zeatin,IAA/JA,ABA/TZR,ABA/Zeatin,ABA/JA in scions and rootstocks were higher in Changlin 53 than in Changlin 18.The ratio of SA/IAA in scions was higher in Changlin 18 than Changlin 53,and it was higher in Changlin 18 than Changlin 53 in ratios of SA/JA,SA/ABA of scions while it was higher in Changlin 18 in s

关 键 词：油茶 芽苗砧嫁接 砧木 穗条 创伤 内源激素 

分 类 号：S603.8[农业科学—园艺学] S794.4[农业科学—林木遗传育种]