白桦BpNAC012基因调控白桦木质部发育表达谱分析  

Expression Profile Analysis of Xylem Development Regulated by BpNAC012 Gene from Betula platyphylla

作　　者：郭依萍 刘佳欣[1] 于颖[1] 王超[1] 杨传平[1] GUO Yi-Ping;LIU Jia-Xin;YU Ying;WANG Chao;YANG Chuan-Ping(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040)

机构地区：[1]林木遗传育种国家重点实验室,东北林业大学,哈尔滨150040

出　　处：《植物研究》2021年第2期251-261,共11页Bulletin of Botanical Research

基　　金：中央高校基本科研业务费(2572016DA01)。

摘　　要：NAC转录因子成员被认为是调控植物次生壁合成的开关。前期研究结果显示BpNAC012基因的表达能够调控白桦次生细胞壁的合成。为研究BpNAC012调控的下游靶基因,本研究分别以该基因的过表达,抑制表达株系茎为材料构建转录组,以野生型为对照分析差异表达基因。结果显示:与对照相比,过表达株系OE中上调的基因有627条,下调的基因有229条,抑制表达株系中上调的基因有299条,下调表达的基因有207条。过表达BpNAC012相对于抑制表达能够调控更多的基因表达变化。而抑制表达BpNAC012更多的是影响蛋白修饰和转运类基因的表达变化。在差异表达基因中,涉及受体信号通路,营养代谢,氨基酸合成,及苯丙烷生物合成相关代谢通路基因比较富集。BpNAC012能够调控纤维素、木质素合成及木质部发育相关基因的表达变化,同时能够调控多种转录因子的表达变化。该研究为深入分析BpNAC012在白桦次生细胞壁合成的分子调控机制奠定基础。NAC transcription factors have been considered to be the switches that regulate the formation of plant secondary cell walls.Our previous work indicated that the gene BpNAC012 was involved in the secondary cell wall biosynthesis of birch.In order to identify the downstream target genes regulated by BpNAC012,transcriptome sequencing of stems from BpNAC012 over-expression and RNAi plants was conducted,and the differentially expressed genes were identified compare to the wild type birch.The results showed that,in contrast to the wild type plants,627 genes were up-regulated and 229 genes were down-regulated in the overexpression transgenic line,and 299 up-regulated genes and 207 down-regulated genes were identified in the RNAi lines.The expression changes of downstream genes in the overexpression transgenic lines were much greater than that in the RNAi plants.Inhibition of BpNAC012 expression significantly affected the expression of genes related to protein modification and transport.The differentially expressed genes were enriched in pathways such as receptor signaling pathways,nutritional metabolism,amino acid synthesis,and phenylpropane biosynthesis.In addition,BpNAC012 can regulate the expression of genes related to cellulose,lignin biosynthesis and xylem development,as well as a variety of transcription factors.This study laid the foundation for further analysis of the molecular regulatory mechanism of BpNAC012 in secondary cell wall bio-synthesis of birch.

关 键 词：白桦 BpNAC012 表达谱分析 木质部发育 

分 类 号：S792.153[农业科学—林木遗传育种]