獐牙菜亚族植物的系统发育分析  

Molecular Phylogeny of Subtribe Swertiinae

作　　者：曹倩 徐隆华[1,3] 王久利 张发起[1] 陈世龙 CAO Qian;XU Long-Hua;WANG Jiu-Li;ZHANG Fa-Qi;CHEN Shi-Long(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Science,Xining 810003;University of Chinese Academy of Sciences,Beijing 100039;Soil and Fertilizer Workstation of Agricultural and Rural Bureau of Ji-nan,Jinan 250002;College of Ecological Environment and Resources,Qinghai Nationalities University,Xining 810007)

机构地区：[1]中国科学院西北高原生物研究所生物适应与进化重点实验室,西宁810003 [2]中国科学院大学,北京100039 [3]山东省济南市农业农村局,土壤肥料站,济南250002 [4]青海民族大学生态环境与资源学院,西宁810007

出　　处：《植物研究》2021年第3期408-418,共11页Bulletin of Botanical Research

基　　金：国家基金委重大国际合作项目(31110103911);青海省科技厅项目(2019-ZJ-976Q)。

摘　　要：獐牙菜亚族(subtribe Swertiinae)是龙胆科(Gentianaceae)中分类处理较困难的一个亚族。为探讨该亚族各属之间和属内的系统关系,选取了该亚族86种及变种,采用ML和BI方法对样本的叶绿体基因mat K和rbc L片段进行分析,构建了该亚族的系统发育树,用马尔科夫蒙特卡洛算法(MCMC)的分子序列贝叶斯分析推算了该亚族的关键演化时间点。结果显示:①龙胆亚族和獐牙菜亚族各自为单系,且互为姐妹类群;②獐牙菜属、假龙胆属、肋柱花属和喉毛花属均不是单系群,各属的种在系统发育树上互有交叉,特别是獐牙菜属的多个种分别聚到不同的支上,与其它属是并系关系;③獐牙菜亚族49个种在约4 Ma开始形成;④分子数据支持何廷农分类系统对于獐牙菜亚属和多枝亚属的属间划分,部分支持多枝亚属下多枝组和宽丝组的划分;⑤异型花属、獐牙菜属、假龙胆属、喉毛花和肋柱花属的属间分类以及獐牙菜属肉根亚属密花组的系统位置仍需进一步讨论。It was difficult to classify the Subtribe Swertiinae in both taxonomical and phylogenetic inGentianaceae. To understand the relationship and phylogeny of intergenetic and intra-genetic of SubtribeSwertiinae,86 species and variants were performed with maximum likelihood(ML)and Bayesian inferencemethods(BI)based on the sequences of chloroplast DNA mat K and rbc L regions. The key evolution periodpoints of Subtribe Swertiinae were estimated through Bayesian analysis of molecular sequences using MCMC.The phylogenetic relationships of Subtribe Swertiinae inferred from molecular evidence were examined againstthe taxonomic circumscriptions in Ho Ting-nong system(2015). The result revealed that:(1) SubtribeGentianinae and Swertiinae were monophyletic and sister groups.(2) Swertia,Gentianella,Comastoma andLomatogonium were paraphyletic in relation to each other.(3) Speciation of 49(85.96%)species occurred insuccession after 4 Ma,and 39(68.42%)species occurred in succession after 2.5 Ma.(4)With respect of intragenetic relationships of Swertia,the taxonomic treatment between subgen. Swertia and subgen. Ophelia weresupported by the phylogenies results,while the taxonomic treatment of sect. Ophelia and sect. Platynema werepartially supported.(5) The intergenetic phylogeny of Sinoswertia,Swertia,Gentianella,Comastoma andLomatogonium need further study,the systematic position of sect. Frasera was still uncertain.

关 键 词：獐牙菜亚族 CPDNA 系统发育 龙胆科 

分 类 号：Q949.776.4[生物学—植物学]