左旋奥拉西坦对小鼠学习记忆功能障碍的影响  

Effects of S-oxiracetam on learning and memory impairment in mice

作　　者：樊文香 李晓敏[1] 徐驰 FAN Wenxiang;LI Xiaomin;XU Chi(Department of Pharmacy,Zhongda Hospital,Southeast University,Nanjing 210009;Department of Neurobiology and Acupuncture Research,The 3rd Clinical College of Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]东南大学附属中大医院药学部,南京210009 [2]浙江中医药大学第三临床医学院针灸神经生物学实验室,杭州310053

出　　处：《中国药科大学学报》2021年第1期77-83,共7页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81701313);中央高校基本科研业务费专项资金资助项目(No.2242020K40175)。

摘　　要：研究左旋奥拉西坦对动物记忆障碍模型的影响,并探讨其可能机制。实验分为空白组、模型对照组、左旋奥拉西坦高剂量(0.96 g/kg)、左旋奥拉西坦中剂量(0.48 g/kg)和左旋奥拉西坦低剂量(0.24 g/kg)组。采用跳台实验和Y-电迷宫实验研究左旋奥拉西坦对东莨菪碱所致小鼠记忆获得障碍、亚硝酸钠所致小鼠记忆巩固障碍和乙醇所致小鼠记忆再现障碍的影响,并用试剂盒检测东莨菪碱所致记忆获得障碍小鼠脑组织中乙酰胆碱(ACh)含量和乙酰胆碱酯酶(AChE)活性。跳台实验结果显示,左旋奥拉西坦高、中剂量组较模型对照组小鼠的反应时间、错误次数均显著性降低,逃避潜伏期显著性延长。Y-电迷宫实验结果显示,与模型对照组相比,左旋奥拉西坦高、中剂量均显著性缩短小鼠的潜伏期,提高正确次数,显著提高小鼠学习记忆保持能力。与东莨菪碱所致小鼠记忆获得障碍模型组相比,左旋奥拉西坦高、中剂量组可显著性升高小鼠脑组织中ACh的含量,降低脑组织中AChE活性。提示左旋奥拉西坦可以改善记忆损伤模型动物的学习记忆能力,其机制可能与升高脑内ACh的含量和降低脑内AChE活性,改善中枢胆碱能神经系统有关。To investigate the effects and possible molecular mechanism of S-oxiracetam(S-ORC)on learning and memory impairment in mice,mice were divided into 5 groups,control group,model group,high-dose of S-ORC(0.96 g/kg),medium-dose of S-ORC(0.48 g/kg)and low-dose of S-ORC(0.24 g/kg)treatment groups.Step-down test and Y-maze test were used to investigate the effects of S-ORC on the brain.The results of step-down test revealed that the mice in high and medium-dose groups could significantly decrease the reaction time,fault times and prolong the incubation periods of memory compared with the model group.Compared with the model group,the fault times of mice in high and medium-dose groups decreased significantly and the right times to find the safety increased significantly in Y-maze test.Furthermore,through treatment with S-ORC(high and medium-dose groups),the content of Ach in mice brain was significantly higher than that in model group,and the level of AChE decreased significantly.The above results suggest that the underlying mechanism of S-ORC on learning and memory impairment in mice may include the amelioration of the central cholinergic nervous system.

关 键 词：左旋奥拉西坦 学习记忆 跳台法 Y-电迷宫 

分 类 号：R965[医药卫生—药理学]