抗草甘膦转基因大豆ZH10-6对几种除草剂的耐受性检测  

Tolerance of Transgenic Glyphosate-Resistant Soybean ZH10-6 to Herbicides

作　　者：陈景超[1] 崔海兰[1] 郭勇[2] 陈萍萍 黄松涛 彭立存 李香菊[1] CHEN Jing-chao;CUI Hai-lan;GUO Yong;CHEN Ping-ping;HUANG Song-tao;PENG Li-cun;LI Xiang-ju(Institute of Plant Protection,Chinese Academy of Agriculture Science,Beijing 100193,China;Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院植物保护研究所,北京100193 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《杂草学报》2020年第4期26-30,共5页Journal of Weed Science

基　　金：转基因生物新品种培育科技重大专项(编号:2019ZX08013002-005)。

摘　　要：为明确抗除草剂转基因大豆ZH10-6对靶标除草剂草甘膦及几种常见除草剂的耐受性水平,在温室隔离条件下培养ZH10-6及其受体大豆中黄10,分别对其喷施不同浓度的茎叶处理剂草甘膦、乙羧氟草醚、精喹禾灵、2,4-滴二甲胺盐和土壤处理剂乙草胺,并对其药害症状、株高、干重等指标进行检测。结果表明:转基因大豆ZH10-6对靶标除草剂草甘膦具有较高的耐受性,在3600 g a.i./hm^(2)处理后28 d,株高、干重与对照无显著差异。而受体大豆中黄10在草甘膦900 g a.i./hm^(2)处理后14 d全部死亡。ZH10-6对大豆田常用除草剂乙羧氟草醚、精喹禾灵和乙草胺也有良好的耐受性,耐受性水平与中黄10无显著差异。转基因大豆ZH10-6及其受体大豆中黄10对2,4-滴二甲胺盐均表现敏感。To determine the tolerance of glyphosate-resistant soybean ZH10-6 to conventional herbicides,soybean ZH10-6 and its receptor line soybean ZH10 were sprayed with the foliage-applied herbicides,glyphosate,fluoroglycofen-ethyl,quizalofop-p-ethyl,2,4-D(dimethylamine salt)at different doses and soil disinfectants under isolated culture conditions in the greenhouse.Based on the development of phytotoxicity symptoms and plant height and dry weight determinations,transgenic soybean ZH10-6 was highly tolerant to the target herbicide,glyphosate.Plants treated with glyphosate at 3600 g a.i./hm^(2)were similar in height and dry weight to those untreated at 28 days after application.Conversely,all ZH10 plants died 14 days after treatment with 900 g a.i./hm^(2)glyphosate.Transgenic soybean ZH10-6 was also tolerant to fluoroglycofen-ethyl,quizalofop-p-ethyl and acetochlor,with no significant difference from ZH10.Both transgenic soybean ZH10-6 and its receptor soybean ZH10 were sensitive to 2,4-D dimethylamine salt.

关 键 词：转基因大豆ZH10-6 草甘膦 常规除草剂 耐受性 

分 类 号：Q142[生物学—生态学]