高粱组ⅢWRKY转录因子对干旱胁迫的表达分析  

Expression Analysis of Group Ⅲ WRKY Transcription Factors in Sorghum Under Drought Stress

作　　者：徐磊[1] 刘洋[1] XU Lei;LIU Yang(Zhanjiang Experiment Station,Chinese Academy of Tropical Agricultural Sciences/Guangdong Engineering Technology Research Center for Dryland Water-Saving Agriculture,Zhanjiang 524013,China)

机构地区：[1]中国热带农业科学院湛江实验站/广东省旱作节水农业工程技术研究中心,广东湛江524013

出　　处：《广东农业科学》2021年第2期11-16,共6页Guangdong Agricultural Sciences

基　　金：中国热带农业科学院基本科研业务费专项资金(1630102017002)。

摘　　要：【目的】组ⅢWRKY基因在植物应对非生物胁迫中起重要调控作用,分析其家族成员响应干旱胁迫的表达谱,为该类基因的生物学功能研究提供参考。【方法】利用NCBI数据库下载高粱基因芯片数据,分析组ⅢWRKY基因响应干旱胁迫、热胁迫和复合胁迫的表达谱;以20%PEG6000模拟干旱胁迫处理高粱品种BTx623幼苗,采用qRT-PCR技术检测基因表达谱。【结果】基因芯片分析结果表明,SbWRKY14、SbWRKY32在干旱胁迫、热胁迫和复合胁迫时下调表达;SbWRKY39在干旱胁迫、热胁迫时下调表达,但在复合胁迫时上调表达;SbWRKY41在3种胁迫处理均上调表达。qRT-PCR检测结果表明,经干旱胁迫处理,SbWRKY10在处理后3、6 h下调表达,而在处理后12 h上调表达;SbWRKY14在处理后1、6、12 h下调表达;SbWRKY16、SbWRKY38、SbWRKY41和SbWRKY42在处理后1、3、6、12 h下调表达;SbWRKY17在处理后1、6、12 h下调表达;SbWRKY39和SbWRKY89在处理后1、3、12 h下调表达;SbWRKY53在处理后1、12 h下调表达,而在处理后6 h上调表达;SbWRKY75在处理后1 h下调表达,而在处理后3、6、12 h上调表达;SbWRKY93在处理后6 h上调表达。【结论】高粱组ⅢWRKY基因可能在高粱应对干旱胁迫中发挥重要作用,不同的SbWRKY基因在应对干旱压力下可能起不同的调控作用。【Objective】Group Ⅲ WRKY genes play an important regulatory role in plant response to abiotic stress.The research was performed to analyze the expression profiles of their family members in response to drought stress so as to provide references for the study of biological function of such genes.【Method】Downloading the microarray data from NCBI database,the expression profiles of group Ⅲ WRKY genes in response to drought,heat and combined stress were analyzed.Seedlings of sorghum variety BTx623 were treated with 20%PEG6000 under simulated drought stress,the expression profiles of these genes were detected by qRT-PCR techniques.【Result】Microarray analysis shows that SbWRKY14 and SbWRKY32 are down-regulated under drought,heat and combined stress.SbWRKY39 is down-regulated under drought and heat stress,but up-regulated under combined stress.SbWRKY41 is up-regulated under three stress treatments.qRT-PCR analysis shows that SbWRKY10 is down-regulated at 3 and 6 h,but up-regulated at 12 h.SbWRKY14 is down-regulated at 1,6 and 12 h.SbWRKY16,SbWRKY38,SbWRKY41 and SbWRKY42 are down-regulated at 1,3,6 and 12 h.SbWRKY17 is down-regulated at 1,6 and 12 h.SbWRKY39 and SbWRKY89 are down-regulated at 1,3 and 12 h.SbWRKY53 is downregulated at 1 and 12 h while up-regulated at 6 h.The expression of SbWRKY75 is down-regulated at 1 h and up-regulated at 3,6 and 12 h.SbWRKY93 is up-regulated at 6 h.【Conclusion】These results indicate that the group Ⅲ WRKY genes of sorghum may play an important role in response to drought stress,and different SbWRKY genes may play different regulatory roles under drought stress.

关 键 词：WRKY基因 转录因子 高粱 干旱胁迫 PEG处理 

分 类 号：S514.01[农业科学—作物学] Q945.78[生物学—植物学]