基于尿液代谢组学的冠心病血瘀证模型大鼠生物学基础研究  

Biological Basic Research on Model Rats of Coronary Heart Disease with Blood Stasis Syndrome Based on Urine Metabolomics

作　　者：李静[1,2] 钟森杰 李亮 吴华英[1,2] 资源[1,3] 袁志鹰 黄惠勇[1,2] LI Jing;ZHONG Senjie;LI Liang;WU Huaying;ZI Yuan;YUAN Zhiying;HUANG Huiyong(Hunan University of Chinese Medicine,Changsha 410208,China;Institute of TCM Diagnostics,Hunan University of Chinese Medicine,Changsha 410208,China;Hengyang Hospital of Traditional Chinese Medicine,Hengyang 421001,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学中医诊断研究所,湖南长沙410208 [3]衡阳市中医医院,湖南衡阳421001

出　　处：《中国中医药信息杂志》2021年第3期63-68,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81373551、81603512、81874429);湖南省自然科学基金(2019JJ40214);湖南省自然科学基金青年基金(2019JJ50446);湖南省教育厅重点项目(19A380);湖南省中医药科研计划重点项目(202004);湖南省研究生科研创新项目(CX20190536、CX20190591)。

摘　　要：目的研究冠心病血瘀证模型大鼠尿液代谢组学特征,探讨其潜在的生物学基础。方法将20只SD大鼠随机分为假手术组和冠心病血瘀证模型组。采用结扎大鼠左冠状动脉前降支方法制备冠心病血瘀证模型。利用超高效液相色谱-质谱联用技术检测假手术组和冠心病血瘀证模型组尿液样本小分子内源代谢物,进行主成分分析和正交偏最小二乘法-判别分析,筛选潜在生物标志物与显著代谢通路。结果与假手术组比较,冠心病血瘀证模型组有36种差异代谢产物水平紊乱,涉及丙氨酸、天冬氨酸和谷氨酸代谢,磷酸戊糖途径,糖酵解/糖异生,泛酸和辅酶A生物合成,柠檬酸盐循环,丙酮酸代谢,嘌呤代谢,甘油脂代谢,戊糖和葡萄糖醛酸酯的相互转化等多条显著代谢通路。结论冠心病血瘀证模型大鼠尿液代谢组学出现改变,其病理机制主要涉及能量代谢失衡,以及氨基酸代谢、糖代谢、嘌呤代谢紊乱及脂质堆积等多个层面。Objectives To study the characteristics of the urine metabolomics of model rats for coronary heart disease blood stasis syndrome,and to explore their potential biological basis.Methods Twenty SD rats were randomly divided into a sham operation group and a coronary heart disease blood stasis syndrome model group.The rats were ligated on the left anterior descending coronary artery to prepare a coronary heart diseases blood stasis model.The UPLC-QE-MS technology was used to detect the endogenous small molecular metabolites in the urine samples of the sham operation group and the coronary heart disease blood stasis syndrome model group.The PCA and the OPLS-DA were used to screen for potential biomarkers and significant metabolic pathways.Results Compared with the sham operation group,the levels of 36 differential metabolites in the coronary disease blood stasis syndrome model group were disordered,involving Alanine,aspartate and glutamate metabolism,Pentose phosphate pathway,Glycolysis/Gluconeogenesis,Pantothenate and CoA biosynthesis,Citrate cycle,Pyruvate metabolism,Purine metabolism,Glycerolipid metabolism,Pentose and glucuronate interconversions,and many other significant metabolic pathways.Conclusion The urine metabolomics of coronary heart disease blood stasis syndrome model rats has changed,and its pathological mechanism mainly involves energy metabolism imbalance,metabolic disorders of amino acids,carbohydrates and purines,lipids accumulation and many other aspects.

关 键 词：冠状动脉粥样硬化性心脏病 血瘀证 中医学 代谢组学 生物学基础研究 

分 类 号：R285.5[医药卫生—中药学]