荚用菜豆松散型胚性愈伤组织保持体系的诱导方法  

Maintain System of Loose Embryonic Callus of Green Bean: Induction Method

作　　者：古瑜 刘艳军[5] 韩启厚[2,3,4] 于海龙[2,3,4] 孙德岭 Gu Yu;Liu Yanjun;Han Qihou;Yu Hailong;Sun Deling(Tianjin Vegetable Research Center,Tianjin 300384;Tianjin Kernel Vegetable Research Institute,Tianjin 300384;State Key Laboratory of Vegetable Germplasm Innovation,Tianjin 300384;Tianjin Key Laboratory of Vegetable Breeding Enterprise,Tianjin 300384;Tianjin Agronomy College,Tianjin 300384)

机构地区：[1]天津市蔬菜研究中心,天津300384 [2]天津科润蔬菜研究所,天津300384 [3]蔬菜种质创新国家重点实验室,天津300384 [4]天津市蔬菜遗传育种企业重点实验室,天津300384 [5]天津农学院,天津300384

出　　处：《中国农学通报》2020年第33期114-119,共6页Chinese Agricultural Science Bulletin

基　　金：天津市科技重大专项与工程计划项目“荚用菜豆新种质创制方法及种质资源创新研究”(17ZXZYNC00030)。

摘　　要：本研究旨在为诱导再生成苗提供基础材料,为进一步开展荚用菜豆育种相关研究奠定基础。以荚用菜豆无性细胞(茎、叶、子叶等器官)为试验材料,通过筛选植物激素及其最佳组合,获得了以细胞质浓密的圆球形细胞为主体的松散型愈伤组织细胞团。最终筛选出诱导无性细胞产生普通愈伤组织的"诱导培养基Ⅰ号":MS+2,4-D 1 mg/L+蔗糖30 g/L。进一步研究获得了"诱导培养基Ⅱ号":MS+6-BA0.25 mg/L+2,4-D 0.5 mg/L+AgNO3 10 mg/L。此培养基可以诱导和保持松散型胚性愈伤组织。本研究筛选出可以将无性细胞诱导为松散型胚性细胞团,并且维持胚性细胞继续增殖的系列培养基,解决了诱导过程中愈伤组织褐化问题,创建了一套诱导荚用菜豆松散型胚性愈伤组织的新方法。The research aims to provide basic materials for the inducing regeneration of seedlings, and lay a foundation for the breeding of green bean(Phaseolus vulgaris L.).Using green bean asexual cells(stem, leaf,cotyledon, etc.) as experiment materials, the loose callus cell clusters which were mainly based on globular cells with dense cytoplasm were obtained by screening phytohormones and their optimum combination.Finally,the"Induction Medium Ⅰ"(MS +2,4-D 1 mg/L+ sucrose 30 g/L) which could induce asexual cells to produce common callus was screened.Moreover, we obtained the"Induction Medium Ⅱ"(MS+6-BA 0.25 mg/L+2,4-D0.5 mg/L+ AgNO3 10 mg/L) which could induce and maintain the loose embryogenic callus.In this research, we screened a series of media which could induce asexual cells to loose embryogenic cell clusters and maintain embryogenic cell proliferation, solved the problem of callus darkening during induction, and established a new method to induce loose embryogenic callus.

关 键 词：荚用菜豆 松散型胚性愈伤 诱导培养基 保持培养基 愈伤褐化 

分 类 号：S643.1[农业科学—蔬菜学]