人源脐带间充质干细胞对膝骨关节炎模型大鼠的治疗作用  

Experimental study of human umbilical cord mesenchymal stem cells in treatment of knee osteoarthritis in rats

作　　者：李伟 李政楠 王秀娟 牟春琳 杨莹 金星 徐永胜 LI Wei;LI Zhengnan;WANG Xiujuan;MOU Chunlin;YANG Ying;JIN Xing;XU Yongsheng(Ever Union Biotechnology Company Limited,Tianjin 301925,China)

机构地区：[1]天津长和生物技术有限公司,天津301925

出　　处：《药物评价研究》2020年第12期2380-2384,共5页Drug Evaluation Research

基　　金：国家重点研发计划“干细胞及转化研究”重点专项(2019YFA0112100);天津市科技计划项目(17ZXXYSY00020)。

摘　　要：目的观察人脐带间充质干细胞(hUC-MSCs)对SD大鼠膝骨关节炎的治疗效果。方法8周龄近交系SD雄性大鼠6只,均采用自身双侧对照研究。应用手术方法切断双膝关节前交叉韧带,手术后不固定伤肢,笼内自由活动。术后4周,右侧作为模型侧,左侧膝关节注射hUC-MSCs 100μL(1×10^(7)cells/mL)。注射后4周,处死所有实验组大鼠并收取双侧膝关节标本,进行HE和甲苯胺蓝染色。结果模型侧细胞排列紊乱,细胞簇集明显,有细胞消失、基质染色不均、软骨结构改变、表面凹凸不平、软骨层次较差等现象,表明软骨蜕变,骨关节炎形成;hUC-MSCs治疗侧与模型侧相比,软骨细胞排列基本整齐,呈柱状,细胞增生较多,细胞簇集明显减少,潮线较完整。软骨表面相对平滑,表明软骨处于损伤修复中。结论hUC-MSCs移植治疗有助于改善大鼠膝骨关节炎,使大鼠关节软骨破坏减轻,促进软骨组织修复。Objective To observe the therapeutic effect of human umbilical cord mesenchymal stem cell injection on knee osteoarthritis in SD rats.Methods Inbred Sprague Dawley(SD)male rats of eight weeks old were studied by bilateral self control.The anterior cruciate ligament of both knee joints was cut by surgical method,the injured limb was not fixed after surgery,and the cage was free to move.Four weeks after the operation,human umbilical cord mesenchymal stem cell injection of 100μL(1×10^(7)cells/mL)was injected into the left knee joint.Four weeks after injection,all rats in the experimental group were sacrificed and bilateral knee specimens were collected.Results HE and toluidine blue staining showed that the cells on the single operation side were disordered,the cell clusters were obvious,the cells disappeared,the matrix staining was uneven,the cartilage structure changed,the surface was uneven,and the cartilage level was poor,indicating that the cartilage was in metamorphosis and osteoarthritis was formed.Compared with the single operation side,the stem cell treatment side showed that chondrocytes were arranged in a basically neat columnar shape,with more cell proliferation,significantly reduced cell clusters,and relatively intact tidal line.The cartilage surface is relatively smooth,indicating that the cartilage isin repair from injury.Conclusion hUC-MSCs transplantation therapy can improve the arthritis of rats,reduce the damage of articular cartilage and repair the cartilage tissue.

关 键 词：脐带间充质干细胞 骨关节炎 HE染色 甲苯胺蓝染色 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]