百日咳鲍特菌红霉素耐药株与敏感株的基因型别分析  

Genotype analysis of erythromycin-resistant and-sensitive Bordetella pertussis

作　　者：张娟胜 魏晓光[1] 王小强[1] 张弟强[2] 李浩[1] 赵悦宛 韩锐郡[1] 李芳[1] 刘莹[1] Zhang Juansheng;Wei Xiaoguang;Wang Xiaoqiang;Zhang Diqiang;Li Hao;Zhao Yuewan;Han Ruijun;Li Fang;Liu Ying(Department of Microbiology and Bioassay,Xi′an Center for Disease Control and Prevention,Xi′an 710054,China;Department of Obstetrics and Gynecology,Pingchuan District People′s Hospital,Baiyin 730900,China)

机构地区：[1]西安市疾病预防控制中心微生物与生物检验科,710054 [2]白银市平川区人民医院妇产科,730900

出　　处：《中华微生物学和免疫学杂志》2020年第12期893-897,共5页Chinese Journal of Microbiology and Immunology

基　　金：西安市科技计划项目(201805101YX9SF35(2));西安卫健委卫生科研人才培训项目(J201801016)。

摘　　要：目的比较百日咳鲍特菌耐药株与敏感株的基因型别,分析百日咳菌株耐药性演变与分子型别特征的关联性。方法于2016—2018年在监测医院和社区采集<1岁临床疑似百日咳患儿的鼻咽拭子,采用E-test法检测分离的百日咳鲍特菌对红霉素的耐药性,扩增23S rRNA基因并测序检测其2047位点耐药突变;采用多位点抗原序列分型(MAST)和多位点可变数目串联重复序列分析(MLVA)方法对菌株进行分型。结果600份鼻咽拭子标本共分离到34株百日咳鲍特菌,其中28株对红霉素耐药,6株敏感。所有红霉素耐药株均检测到23S rRNA基因A2047G位点突变,细菌基因型均为prn1/ptxP1/ptxA1/fim3-1/fim2-1,且MLVA型别识别为MT195、MT55和MT104。6株敏感株均未检测到A2047G位点突变,细菌基因型为prn9(或prn2)/ptxP3/ptxA1/fim3-1/fim2-1,且MLVA型别全部为MT27型。结论百日咳鲍特菌红霉素耐药株与敏感株的分子分型特征不同,红霉素耐药性的获得可能伴随特定分子型别的变化。Objective To compare the genotypes of erythromycin-resistant and-sensitive Bordetella pertussis,and analyze the relationship between the evolution of drug resistance and the molecular characteristics of Bordetella pertussis.Methods Nasopharyngeal swabs were collected from patients<1 year old with suspected pertussis infection in the disease monitoring hospitals and community in Xi′an during 2016 to 2018.Erythromycin susceptibility of Bordetella pertussis isolates was tested by E-test.A fragment of 23S rRNA gene was amplified and sequenced to detect drug resistance mutations at the 2047 site.Genotypes of the isolated strains were analyzed with multilocus antigen sequence typing(MAST)and multiple locus variable-number tandem repeat analysis(MLVA).Results A total of 34 Bordetella pertussis strains were isolated from 600 nasopharyngeal swabs.Among them,28 were resistant to erythromycin and six were sensitive strains.All erythromycin-resistant strains carried a genetic mutation at the A2047G site in the 23S rRNA gene and belonged to the genotype of prn1/ptxP1/ptxA1/fim3-1/fim2-1.Three MLVA types,MT195,MT55 and MT104,were identified in these strains.However,no mutation at the A2047G site was found in the six erythromycin-sensitive strains whose genotypes were prn9(or prn2)/ptxP3/ptxA1/fim3-1/fim2-1 and MT27.Conclusions There were differences between erythromycin-resistant and-sensitive Bordetella pertussis in genotypes,suggesting that erythromycin resistance might be associated with molecular types.

关 键 词：百日咳鲍特菌 红霉素 耐药 基因型 分子流行病学 

分 类 号：R378.42[医药卫生—病原生物学]