大豆硬实性QTL定位及上位性互作分析  

QTL mapping and epistatic interaction analysis of hard seededness in soybean seed

作　　者：滕卫丽[1] 李悦[1] 郑立娜 郭志文 付雪 王博[1] 董莹莹 韩英鹏[1] 李文滨[1] TENG Weili;LI Yue;ZHENG Lina;GUO Zhiwen;FU Xue;WANG Bo;DONG Yingying;HAN Yingpeng;LI Wenbin(Institute of Soybean Research,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Chinese Agriculture and Rural Ministry,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学大豆研究所,大豆生物学教育部重点实验室,农业农村部东北农业大学生物学与遗传育种重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2021年第1期8-19,共12页Journal of Northeast Agricultural University

基　　金：黑龙江省“百千万”工程科技重大专项课题(2019ZX16B01-1);国家自然科学基金项目(31471517);现代农业产业技术体系建设专项资金项目(CARS-04-PS04)。

摘　　要：试验以东农46和L-100杂交构建的包含127个家系F_(2:10)、F_(2:11)、F_(2:12)代重组自交系群体为试验材料,结合三年五点以及4个浸种时间段,调查大豆硬实率和种子吸水量两个表型性状,采用QTL IciMapping 4.1完备区间作图法,作QTL定位和加性效应分析。结果共检测到26个与大豆硬实性相关QTLs,分布于10条染色体,贡献率5.29%~47.31%,有8个位点在两个性状QTL定位中发生标记区间重叠,分别为qHS-2-1和qWAS-2-1、qHS-2-2和qWAS-2-2、qHS-4-2和qWAS-4-1、qHS-13-1和qWAS-13-3,其中,qHS-2-2和qWAS-2-2贡献率平均值最大,>25%。共检测到51对加性×加性上位互作QTL,贡献率为1.01%~8.40%。In this study,127 lines of F_(2:10),F_(2:11) and F_(2:12) recombinant inbred lines constructed by Dongnong 46 and L-100 were used as the materials and the two phenotypic traits related to soybean hard seededness were investigated,combined with three years,five sites and four seed soaking times.The inclusive complete interval mapping(ICIM)method in IciMapping 4.1 software adopted for QTL mapping and analysis of additive effect.The results showed 26 QTLs related to soybean hard seededness were detected on 10 chromosomes,with the contribution rate ranging from 5.29%to 47.31%.Eight QTL loci,qHS-2-1 and qWAS-2-1,qHS-2-2 and qWAS-2-2,qHS-4-2 and qWAS-4-1,qHS-13-1 and qWAS-13-3,overlapped in the two traits,respectively.The average contribution rate of qHS-2-2 and qWAS-2-2 was the highest,which was greater than 25%.A total of 51 QTLs for additive×additive epistatic interactions were detected,and the range of the contribution rate was 1.01%-8.40%.

关 键 词：大豆 硬实率 种子吸水量 硬实性 QTL 

分 类 号：S451.21[农业科学—植物保护]