左金丸醇提物抑制人胃癌SGC-7901细胞糖酵解的作用机制  

Mechanism of alcohol extract of Zuojin Pills on inhibiting glycolysis in SGC-7901 cells

作　　者：吴秋雪 孙梦瑶 许博[1] 张辉[2] 汤庆丰[1] WU Qiu-xue;SUN Meng-yao;XU Bo;ZHANG Hui;TANG Qing-feng(Putuo Hospital,shanghai University of Traditional Chinese Medicine,Shanghai 200062,China;Shanghai University of Traditional Chinese Medicine,Shanghai 200135,China)

机构地区：[1]上海中医药大学附属普陀医院,上海200062 [2]上海中医药大学,上海200135

出　　处：《中草药》2021年第1期145-151,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81973808);上海市自然科学基金资助项目(19ZR1447800);上海中医药大学预算内项目(2019LK038)。

摘　　要：目的研究左金丸醇提物对人胃癌细胞SGC-7901增殖和糖酵解的作用及机制。方法采用CCK-8法检测左金丸醇提物(5、10、25、50、100、200μg/mL)对SGC-7901细胞增殖的影响;检测左金丸醇提物对SGC-7901细胞葡萄糖摄取量、乳酸和三磷酸腺苷(adenosine triphosphate,ATP)含量的影响,评价左金丸醇提物对SGC-7901细胞糖酵解的作用;采用qRT-PCR和Western blotting法检测左金丸醇提物对细胞糖酵解途径相关蛋白Rhotekin(RTKN)表达的影响;采用RTKN shRNA干扰慢病毒感染SGC-7901细胞,检测左金丸醇提物对SGC-7901细胞葡萄糖摄取、乳酸和ATP含量的影响。结果左金丸醇提物对SGC-7901细胞增殖具有显著抑制作用(P<0.001),呈剂量、时间相关性;左金丸醇提物显著降低SGC-7901细胞葡萄糖摄取、乳酸和ATP含量(P<0.01、0.001);左金丸醇提物显著降低SGC-7901细胞中RTKN mRNA和RTKN蛋白表达(P<0.001);干扰RTKN抵消了左金丸醇提物对SGC-7901细胞葡萄糖摄取、乳酸和ATP含量的抑制作用(P<0.001)。结论左金丸醇提物可显著抑制SGC-7901细胞增殖、降低细胞糖酵解水平,其机制与下调RTKN表达相关。Objective To investigate the effect of Zuojin Pills(左金丸)alcohol extract on proliferation and glycolysis of gastric cancer SGC-7901 cells by downregulating Rhotekin(RTKN).Methods CCK-8 was used to evaluate the effect of Zuojin Pills alcohol extract(10,25,50,100,200μg/m L)on cell viability of SGC-7901 cell.Glucose uptake,lactate and ATP content of SGC-7901 cells were detected to evaluate the effects of Zuojin Pills alcohol extract on glycolysis of SGC-7901 cells.qRT-PCR and Western blotting were used to detect glycolysis related protein RTKN expression.Moreover,RTKN sh RNA were transfected into SGC-7901 cells to knockdown RTKN and the effects of Zuojin Pills alcohol extract on glucose uptake,lactate and ATP content were evaluated.Results Zuojin Pills alcohol extract exhibited a dose-and time-dependent anti-proliferative effect on SGC-7901 cells(P<0.001).In addition,glucose uptake,lactate and ATP content of SGC-7901 cells were significantly decreased(P<0.01,0.001)with Zuojin Pills alcohol extract treatment.Zuojin Pills alcohol extract significantly reduced RTKN m RNA and RTKN protein expression in SGC-7901 cells(P<0.001).However,RTKN knockdown significantly attenuated the inhibitory effect of Zuojin Pills alcohol extract on glycolysis of SGC-7901 cells(P<0.001).Conclusion Zuojin Pills alcohol extract significantly inhibited the proliferation and glycolysis of SGC-7901 cells,which was related to the downregulation of RTKN.

关 键 词：左金丸 胃癌 细胞增殖 糖酵解 Rhotekin 

分 类 号：R285.5[医药卫生—中药学]