芬兰贝氏藻(硅藻门)的超微结构研究  

Ultrastructure of Berkeleya fennica Juhlin-Dannfelt(Bacillariophyta)

作　　者：龚志港 刘冰[1] 刘祝祥[1] 陈锦华 袁莉 GONG Zhigang;LIU Bing;LIU Zhuxiang;CHEN Jinhua;YUAN Li(College of Biology and Environmental Science, Jishou University, Jishou, Hunan 416000, China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《西北植物学报》2020年第12期2075-2080,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31760051)。

摘　　要：以采集于青海湖的石附生硅藻(芬兰贝氏藻)为样品,用10%的HCl溶液溶解样品中含钙的固体化合物,再加入30%H2O2溶液氧化去掉硅藻细胞的有机物质,获得干净的硅藻标本。利用Naphrax封装剂制成永久装片,在光学和电子显微镜下对其形态特征进行了详细研究。结果表明:(1)在光学显微镜下芬兰贝氏藻具有披针形的壳瓣外形、尖圆的壳面末端和明显伸长的中轴区三个特征。(2)在扫描电子显微镜下芬兰贝氏藻紧邻中央区的孔纹明显伸长,其长度为2~4个正常孔纹的长度。(3)通过文献查阅和相似种类的比较发现,同时具有尖圆的末端和明显伸长的紧邻中央区的孔纹是芬兰贝氏藻鉴定的独特性状组合,故芬兰贝氏藻的身份可由此确定。该研究首次报道了芬兰贝氏藻在中国的分布。The material of an epilithic diatom sample(including Berkeleya fennica),collected from Lake Qinghai,was processed using 10%HCl to dissolve all solid compound containing calcium,and then adding 30%H2O2 to oxidize and remove the organic matter of diatom cells.As a result,the clean diatom specimens can finally be obtained.Permanent slides were prepared well by using Naphraxmountant.The morphological characters of B.fennica were thoroughly studied under light and scanning electron microscope.The results show:(1)under light microscope,B.fennica possesses three characters:lanceolate valve outline,acutely rounded valve apices and markedly elongated central area.(2)Under scanning electron microscope,B.fennica possesses markedly elongated areolae which are close to the central area,and their lengths are equal to the ones of 2-4 normal areolae.(3)After consulting the literature and making comparisons between similar taxa,the current paper can confirm that the unique identifying character combination of B.fennica is the combination of the acutely rounded valve apices and the markedly elongated areolae close to the central area,by which the identity of B.fennica can be confirmed.Berkeleya fennica is reported for the first time in China in this paper.

关 键 词：青海湖 咸水硅藻 贝氏藻属 中央区 伸长的孔纹 

分 类 号：Q949.27[生物学—植物学]