三带喙库蚊微卫星标记的筛选及遗传多样性分析  

ISOLATION AND GENETIC DIVERSITY ANALYSIS OF MICROSATELLITE MARKERS IN CULEX TRITAENIORHYNCHUS(DIPTERA:CULICIDAE)

作　　者：褚宏亮[1] 吴治明[1] 田野[1] 高剑 周明浩[1] 赵彤言 CHU Hong-Liang;WU Zhi-Ming;TIAN Ye;GAO Jian;ZHOU Ming-Hao;ZHAO Tong-Yan(Center for Disease Control and Prevention of Jiangsu Province,Nanjing 210009,China;Beijing Key Laboratory of Vector Borne and Natural Infectious Disease,State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,AMMS,Beijing 100071,China)

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009 [2]军事科学院军事医学研究院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京市虫媒病与自然疫源性疾病重点实验室,北京100071

出　　处：《寄生虫与医学昆虫学报》2020年第4期218-222,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：江苏省青年医学人才(QNRC2016544);江苏省卫生健康委面上课题(H2018103)。

摘　　要：为了便于对我国三带喙库蚊种群的遗传结构和多样性进行动态检测,本研究利用磁珠富集法进行三带喙库蚊微卫星标记的开发和筛选,共获得40个微卫星位点,且位点的多态性验证结果表明:有24个位点在不同三带喙库蚊地理株间呈现多态性。微卫星位点的遗传多样性分析结果表明:各位点的平均多态信息含量(PIC)和香农指数(SI)均较高,其中位点P17最高(PIC=0.853,SI=2.281),最低的为位点P40(PIC=0.503,SI=1.195),都属于高度多态性位点,适用于后续三带喙库蚊种群遗传多样性及遗传结构的研究。To monitor the dynamic changes of genetic diversity and population structure of Culex tritaeniorhynchus populations in China,a total of 40 microsatellite loci were developed from its genome via Magnetic Beads Enrichment method.The polymorphism verification result showed that 24 loci of all displayed polymorphic among Cx.tritaeniorhynchus populations from different geographic regions.Meanwhile,genetic diversity analysis results of these microsatellite loci showed that both of the average polymorphic information content(PIC)and Shannon index(SI)of these loci were high,with the highest one was locus P17(PIC=0.853,SI=2.281)and lowest one was locus P40(PIC=0.503,SI=1.195).Therefore,these new developed microsatellite loci are suitable for the subsequent study of the genetic diversity and population structure of Cx.tritaeniorhynchus.

关 键 词：三带喙库蚊 日本脑炎病毒 磁珠富集法 微卫星标记 遗传多样性 

分 类 号：R384.1[医药卫生—医学寄生虫学]