荧光PCR法检测2008年-2017年单纯疱疹病毒Ⅱ型感染的临床结果与分析  

Detection and analysis of herpes simplex virus type Ⅱ infection in 2008-2017 by fluorescence PCR

作　　者：葛玉梅[1] 叶春梅 董晓燕 赵钊[1] GE Yu-mei;YE Chun-mei;DONG Xiao-yan;ZHAO Zhao(Clinical Laboratory Medicine,Zhejiang Provincial People's Hospital,People's Hospital Affiliated to Hangzhou Medical College,Hangzhou,Zhejiang 310014,China;不详)

机构地区：[1]浙江省人民医院临床医学检验中心,杭州医学院附属人民医院,浙江杭州310014 [2]浙江中医药大学医学技术学院,浙江杭州310053 [3]浙江中医药大学第二临床医学院,浙江杭州310053

出　　处：《中国卫生检验杂志》2021年第1期1-3,9,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金青年基金项目(81802021);浙江省医药卫生科技项目(2017KY004,2019KY013);国家卫生和计划生育委员会科研基金(WKJ-ZJ-1604);湖州市科技计划项目(2017GY55)。

摘　　要：目的了解浙江地区疑似生殖器疱疹患者单纯疱疹病毒Ⅱ型(HSV-Ⅱ)感染情况,为临床诊断治疗提供依据。方法实时荧光PCR法对浙江省人民医院2008年-2017年2 230例疑似泌尿生殖道疱疹患者进行HSV-Ⅱ核酸检测。结果在2 230例疑似泌尿生殖道疱疹患者中,女性1 554例,男性676例,共检测出HSV-Ⅱ型阳性557例,阳性率为24.98%。557例HSV-Ⅱ感染患者中女性405例,阳性率为26.06%;男性152例,阳性率为22.49%,男、女感染率差异无统计学意义(P> 0.05)。557例HSV-Ⅱ型阳性患者分5个年龄段即<20岁、20岁~29岁、30岁~39岁、40岁~49岁、≥50岁分析,阳性率分别为3.05%、42.01%、29.80%、14.72%、10.23%。结论泌尿生殖道HSV-Ⅱ感染检出率较高,提示临床需重视对疑似生殖道疱疹病毒感染患者进行HSV-Ⅱ核酸检测;HSV-Ⅱ感染无性别差异,但感染主要集中在20岁~29岁患者。实时荧光PCR法在HSV-Ⅱ检测中具有快速、灵敏度高、简便等优点,适用于临床检测。Objective To investigate the suspected patients with genital herpes simplex virus type Ⅱ infection in Zhejiang,in order to provide reference for clinical prevention and control. Methods Real-time fluorescent quantitative PCR was used to test herpes simplex virus type Ⅱ nucleic acid of 2 230 suspected outpatients with genital herpes from 2008 to 2016 in Zhejiang Provincial People’s Hospital. Results The positive rate of 2 230 outpatients with suspected genital herpes,including 1 554 female cases and 676 male cases,was 24. 98% with HSV-Ⅱ. The positive rate was 26. 06% among females;male positive rate was 22. 49%,and there was no statistical significance on the difference of the positive detection rate between male and female( P > 0. 05). Five age groups were age < 20 years,20-29 years,30-39 years,40-49 years,> 50 years,the positive detection rate of this five age groups was 3. 05%,42. 01%,29. 80%,14. 72%,10. 23%. Conclusion The detection rate of HSV-Ⅱ in the outpatients with suspected genital herpes infection was high in Zhejiang,indicating that clinical diagnosis should pay attention to the detection of HSV-Ⅱ nucleic acid in outpatients with suspected genital herpes virus. There was no difference of HSV-Ⅱ infection between male and female,but the highest positive detection rate of HSV-Ⅱ DNA was among 20-29 years old. Real-time quantitative PCR was compatible for clinical application with its features of high speed,high sensitivity,simplicity in the detection of HSV-Ⅱ.

关 键 词：单纯疱疹病毒Ⅱ型 实时荧光PCR 泌尿生殖道 

分 类 号：R752.1[医药卫生—皮肤病学与性病学]