三七三醇皂苷对大鼠离体心脏缺血再灌注损伤的作用研究  

Effects of panaxatriol saponins on ischemia-reperfusion injury in isolated-rat heart

作　　者：文超 王子杨 鲁娜 鲁卫星 Wen Chao;Wang Ziyang;Lu Na;Lu Weixing(Department of Cardiology,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;the Second Clinical Medical College,Beijing University of Chinese Medicine,Beijing 100029,China;Department of Medical Insurance,Zhangqiu District People's Hospital,Jinan 250200,China)

机构地区：[1]北京中医药大学第三附属医院心内科,100029 [2]北京中医药大学第二临床医学院,100029 [3]济南市章丘区人民医院医保科,250200

出　　处：《中国医药》2021年第2期297-301,共5页China Medicine

基　　金：北京市自然科学基金(7172124)。

摘　　要：目的建立Langendorff离体心脏局部缺血再灌注(IR)模型,探讨三七三醇皂苷对大鼠离体心脏IR损伤的作用。方法将40只健康雄性Wistar大鼠随机分为对照组、IR模型组和低、中、高剂量三七三醇皂苷组(PTSL组、PTSM组、PTSH组),各8只。对照组只穿线不结扎冠状动脉左前降支近分支,以K-H液持续灌流105 min;IR模型组穿线结扎冠状动脉左前降支近分支处30 min后开放结扎线,K-H液复灌75 min;PTSL组、PTSM组、PTSH组穿线结扎冠状动脉左前降支近分支处30 min后,分别给予含5、10、20 mg/L三七三醇皂苷的K-H液复灌75 min。收集5组大鼠平衡灌注20 min、局部缺血30 min和再灌注15、75 min的灌流液,检测肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)水平。比较5组平衡灌注20 min、局部缺血30 min和再灌注15、75 min通过多导生理仪记录的心脏血流动力学参数水平,包括心率、左心室收缩末压(LVESP)、左心室舒张末压(LVEDP)、左心室发展压(LVDP)、左心室内压上升/下降最大速率(±dp/dt max),以及LVESP、LVDP、+dp/dt max与平衡末差值占比。结果IR模型组再灌注15、75 min灌流液中CK-MB和LDH水平均高于对照组[CK-MB:(234±51)U/L比(12±6)U/L,(203±63)U/L比(14±3)U/L;LDH:(63.9±9.4)U/L比(6.2±4.6)U/L,(52.1±9.5)U/L比(6.3±4.8)U/L];PTSL、PTSM、PTSH组再灌注75 min灌流液CK-MB水平和再灌注15 min LDH水平均低于IR模型组,PTSL、PTSH组再灌注75 min LDH水平均低于IR模型组(均P<0.05)。对照组不同时点灌流液心肌酶指标水平比较差异均无统计学意义(均P>0.05)。与对照组比较,IR模型组再灌注15、75 min LVDP、+dp/dt max均降低,LVEDP、-dp/dt max以及LVESP、LVDP、+dp/dt max与平衡末差值占比均升高(均P<0.05)。与IR模型组比较,PTSL组、PTSM组、PTSH组再灌注15 min LVEDP,PTSL组再灌注75 min LVEDP均降低,PTSM组再灌注75 min LVDP、+dp/dt max均升高,局部缺血30 min、再灌注75 min-dp/dt max均降低,PTSL组、PTSM组、PTSHObjective To establish a model of local ischemia-reperfusion(IR)in Langendorff isolatedheart and to investigate the effect of panaxatriol saponins(PTS)on ischemia-reperfusion injury in isolated-rat heart.Methods Totally 40 healthy male Wistar rats were divided into the control group,the IR model group and low,medium and high dose PTS groups(PTSL group,PTSM group,PTSH group),with 8 rats in each group.In the control group,proximal branch of the left anterior descending coronary artery was threaded without ligaturing.K-H buffer was continuously reperfused for 105 min.In the IR model group,proximal branch of the left anterior descending coronary artery was threaded and ligatured for 30 min,and then the ligation was loosened.K-H buffer was reperfused for 75 min.In PTSL group,PTSM group and PTSH group,the proximal branch of the left anterior descending coronary artery was threaded and ligatured for 30 min,and K-H buffer respectively containing 5,10 and 20 mg/L PTS were reperfused for 75 min.The levels of creatine kinase isoenzyme(CK-MB)and lactate dehydrogenase(LDH)were measured by collecting the perfusate of balanced perfusion for 20 min,local ischemia30 min and reperfusion for 15 and 75 min in 5 groups.The cardiac hemodynamic parameters recorded by polysomnography including heart rate,left ventricular end systolic pressure(LVESP),left ventricular end diastolic pressure(LVEDP),left ventricular development pressure(LVDP),maximum rate of left ventricular pressure rise/fall(±dp/dt max)and the ratios of LVESP,LVDP,+dp/dt max to final equilibrium difference value were compared among 5 groups at balanced perfusion for 20 min,focal ischemia for 30 min and reperfusion for 15 and75 min.Results At reperfusion for 15,75 min,the levels of CK-MB and LDH in perfusate of the IR model group were higher than those in the control group[CK-MB:(234±51)U/L vs(12±6)U/L,(203±63)U/L vs(14±3)U/L;LDH:(63.9±9.4)U/L vs(6.2±4.6)U/L,(52.1±9.5)U/L vs(6.3±4.8)U/L](all P<0.05).The levels of CK-MB at the reperfusion for 75 min and LDH at the

关 键 词：缺血再灌注 三七三醇皂苷 Langendorff离体心脏 

分 类 号：R542.2[医药卫生—心血管疾病]