镉积累差异水稻品种生长发育对清除NO的响应  

Response of growth and development of rice cultivars with different cadmium accumulation to removing NO

作　　者：曾晶[1] 周旋 荣湘民[1] 张振华[1] ZENG Jing;ZHOU Xuan;RONG Xiang-min;ZHANG Zhen-hua(College of Resource and Environment/College of Oriental Science and Technology,Hunan Agricultural University,Changsha 410128,China;Hunan Institute of Soil and Fertilizer,Changsha 410125,China)

机构地区：[1]湖南农业大学资源环境学院/东方科技学院,湖南长沙410128 [2]湖南省土壤肥料研究所,湖南长沙410125

出　　处：《植物营养与肥料学报》2021年第2期322-331,共10页Journal of Plant Nutrition and Fertilizers

基　　金：国家重点研发计划项目(2018YFD0800500);国家自然科学基金区域创新发展联合基金重点项目(U19A2050)。

摘　　要：【目的】研究一氧化氮(NO)对镉(Cd)胁迫下水稻苗期生理生化及氮代谢响应的调节作用,探讨通过清除NO提高水稻耐Cd能力的措施。【方法】以Cd高积累(TN1)和Cd低积累(春江06)品种为材料,进行了Cd胁迫(40μmol/L)水培试验。以Cd胁迫营养液为对照,在对照基础上设置添加硝普钠(Cd+SNP)、添加NO清除剂CPTIO(Cd+CPTIO)、添加硝酸还原酶抑制剂钨酸钠(Cd+TU)处理,共4个处理。处理后12天,测定不同处理对水稻幼苗生长、NO_(3)^(-)-N分配、根系特性及光合特性的影响。【结果】NO对高Cd和低Cd积累水稻品种的影响不同。与对照相比,Cd+SNP处理降低高Cd积累水稻品种幼苗地上部和地下部NO_(3)^(-)-N含量,但对幼苗生物量和氮素吸收没有显著抑制效果;Cd+CPTIO处理会提高幼苗生长、氮素积累量及氮效率(P<0.05);增加或清除NO处理均显著降低了地上部和地下部的Cd含量。对于低Cd积累水稻品种,Cd+SNP、Cd+CPTIO、Cd+TU处理间幼苗生物量、氮素吸收量均无显著差异。【结论】品种基因型对NO信号的调节敏感度不同,高Cd积累品种清除NO处理可以显著抑制Cd的吸收和向地上部的运转,促进幼苗的生长和对氮素的吸收利用,抑制硝酸还原酶活性处理缓解Cd胁迫促进幼苗生长的效果不稳定。而对于低Cd积累水稻品种,清除NO处理也有一定的缓解Cd吸收和向地上运转的效果,但其重要性不如基因本身的拒Cd能力。因此,Cd胁迫下,高Cd积累水稻品种采用清除NO的措施可有效缓解Cd胁迫,促进水稻的生长,但对于低Cd积累水稻品种,清除NO也可以进一步降低Cd的向上运输,但是对水稻幼苗生长和氮素利用的影响不显著。【Objectives】Nitric oxide(NO)is an active signal molecule that can influence plants response to various stress.This study evaluates the regulatory effect of NO on the physiological,biochemical and nitrogen metabolism of rice seedlings under Cd stress.【Methods】Rice cultivars of high and low Cd accumulation(TN1 and Chunjiang 06)were investigated in Cd stress(40μmol/L)hydroponic trials.The treatments included Cd stress solution(i.e.,control),the addition of sodium nitroprusside to increase NO content(Cd+SNP),the addition of CPTIO to remove NO(Cd+CPTIO),and the addition of NR inhibitor to inhibit NO(Cd+TU).Data was collected on NO_(3)^(-)-N distribution,root development,photosynthetic characteristics,and growth of the rice seedlings.【Results】The effects of NO on the rice varieties with high-and low-Cd accumulation was different(P<0.05).1)The cultivar with high-Cd accumulation recorded low NO_(3)^(-)-N content in the aboveground and underground parts of the rice seedlings,however,no inhibition effect was found in the biomass and N uptake of the seedlings in response to NO regulation in Cd+SNP treatment.Cd+CPTIO treatment increased growth,N accumulation,and N use efficiency of rice seedling(P<0.05).The addition and elimination of NO significantly reduced the Cd contents in the aboveground and underground parts of rice seedlings.2)The treatments(Cd+SNP,Cd+CPTIO and Cd+TU)had no significant effect on seedling growth,N uptake of the low-Cd accumulation cultivar.【Conclusions】The rice cultivar with high Cd accumulation is sensitive to NO regulation but not the cultivar with low Cd accumulation.For the high Cd rice cultivar,removing NO inhibits the absorption and movement of Cd from the root to the shoot,but promotes the absorption and utilization of N and the growth of seedlings.The effect of inhibiting nitrate reductase activity concerning the alleviation of Cd stress to promote seedling growth was instability.For the low Cd accumulation rice cultivar,the elimination of NO has a certain effect on alleviatin

关 键 词：水稻基因型 镉胁迫 NO信号 NO清除剂 NR还原酶抑制剂 

分 类 号：S511[农业科学—作物学]