转录组测序解析刺参波里氏囊腔与体腔中体腔细胞对吐脏胁迫的响应差异  

Transcriptome analysis provides insights into the response of coelomocytes in polian vesicle and coelomic cavity of sea cucumber Apostichopus japonicus to evisceration

作　　者：史伟卜 王轶南 王浩文 任媛 王庆奎 李强 Shi Weibo;Wang Yi’nan;Wang Haowen;Ren Yuan;Wang Qingkui;Li Qiang(College of Marine and Biology Engineering,Yancheng Institute of Technology,Yancheng 224051,China;College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]盐城工学院海洋与生物工程学院,江苏盐城224051 [2]天津农学院水产学院,天津300384 [3]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《海洋学报》2021年第2期116-125,共10页

基　　金：国家自然科学基金(31872544);盐城工学院人才引进项目(XJ201725,XJ201726)。

摘　　要：刺参的体腔细胞大量存在于体腔液与水管系统内,广泛参与机体的营养输送、代谢以及免疫等多种功能。刺参吐脏时,体腔中体腔细胞近乎排尽,而后迅速恢复。波里氏囊是刺参吐脏后仅存的内脏器官,囊腔内体腔细胞在吐脏后也快速增加,表现出积极的响应。为研究探讨刺参吐脏后再生早期体腔细胞快速增加的作用与意义,本文分别对刺参吐脏后6 h与吐脏前波里氏囊腔和体腔中体腔细胞进行转录组测序,比较了波里氏囊腔与体腔中体腔细胞在吐脏胁迫下的基因表达变化。结果显示,波里氏囊腔内体腔细胞在吐脏前后有267个基因显著差异表达,差异基因大量富集至GO功能注释的酶催化活性亚类以及甘氨酸、丝氨酸与苏氨酸代谢等KEGG通路。体腔中体腔细胞在吐脏前后有922个基因显著差异表达,差异基因显著富集到GO功能注释的细胞黏附、生物黏附等亚类以及细胞外基质受体互作、转化生长因子-β与FoxO等KEGG通路。研究结果对于进一步研究刺参体腔细胞的功能及揭示刺参吐脏后的再生机制提供了重要基础。Coelomocytes in Apostichopus japonicus,present in coelomic fluid and water-vascular system,are considered to participate in a variety of biological functions including nutrition transport,metabolism and immunity.In the process of evisceration,coelomocytes in coelom are nearly exhausted and then recovered in a short period.The polian vesicle,as the only remained internal organ after evisceration,shows positive response that coelomocytes within it increased rapidly.Coelomocytes in coelom are nearly exhausted after evisceration,and then recovered quickly.To further investigate the function and significance of the rapid increase of coelomocytes in the early stage of regeneration after evisceration,transcriptome sequencing was performed for coelomocytes in polian vesicle and coelom of A.japonicus at 6 h after evisceration and pre-evisceration,respectively.The gene expression differences of coelomocytes in polian vesicle and coelom after evisceration were analyzed compared to those of healthy A.japonicus.These results showed that 267 genes were differentially expressed in coelomocytes of polian vesicle at 6 h after evisceration,and most of these genes were enriched into the enzyme catalytic activity subclasses according to GO functional annotation and glycine,serine and threonine metabolism pathway according to KEGG pathways annotation,respectively.Additionally,922 differential genes were significantly expressed in coelomocytes of coelom at 6 h after evisceration,and these genes were enriched into cell adhesion and biological adhesion subclasses according to GO functional annotation and ECM-receptor interaction,TGF-βsignaling pathway,FoxO signaling pathway according to KEGG pathways annotation,respectively.The results provide an important basis for further functional research and the regeneration mechanism of coelomocytes after evisceration in A.japonicus.

关 键 词：刺参 体腔细胞 波里氏囊 吐脏 转录组 

分 类 号：S917.4[农业科学—水产科学]