盐地碱蓬SsDHN基因的功能分析  

Functional Analysis of the SsDHN Gene in Suaeda salsa(L.)Pall.

作　　者：马慧[1] 孙华卿 李会 邓珊 王小安 陈水森 陈丽静[1] 张丽[1] 钟鸣[1] Ma Hui;Sun Huaqing;Li Hui;Deng Shan;Wang Xiaoan;Chen Shuisen;Chen Lijing;Zhang Li;Zhong Ming(The Key Laboratory of Agricultural Biotechnology of Liaoning Province,College of Biological Science and Technology,Shenyang Agricultural Uni-versity,Shenyang,110000)

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,沈阳110000

出　　处：《分子植物育种》2021年第3期827-832,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(31070448)资助。

摘　　要：脱水素(DHN)是一种在逆境下起重要作用的功能性蛋白。为了分析脱水素蛋白在抗盐碱方面的作用机制,本研究以从盐地碱蓬中克隆获得的脱水素(Ss DHN)基因为研究对象,对基因序列进行生物信息学分析,结果表明盐地碱蓬的脱水素蛋白为部分无序的亲水性蛋白,含1个S-片段和3个K-片段,属于SKn型脱水素蛋白。通过实时荧光定量PCR分析,结果表明碱蓬SsDHN基因受盐胁迫诱导表达。实验结果证明了Ss DHN蛋白通过自身的无序性,以及S、K片段等结构特性来抵抗盐胁迫。本研究为进一步深入研究SsDHN基因的抗逆机制提供了基础。Dehydrin(DHN)is a functional protein that plays an important role in adversity.In this study,the Dehydrin gene(SsDHN)was cloned from S.salsa(L.)Pall.,and bioinformatics analysis revealed that Ss DHN was partly hydrophilic protein,containing a S-fragment and 3 K-fragment,belonging to SKn-type dehydrin protein.The expression of SsDHN gene through RT-qPCR showed that gene was induced by salt.Our results suggested that Ss DHN protein can resist salt stress through its disorder,and its structural characteristics such as S,K fragment.This study provides a basis for further study on the resistance mechanism of SsDHN gene.

关 键 词：盐地碱蓬(Suaeda salsa) SsDHN 生物信息学分析 实时荧光定量PCR 

分 类 号：Q943.2[生物学—植物学]