基于网络药理学方法探讨八珍汤治疗ITP的作用机制  

Mechanisms of Bazhen Decoction in Treatment of Primary Immune Thrombocytopenia Based on Network Pharmacology

作　　者：姜云耀 刘楠 胡晓梅[3] 侯金才 刘建勋[3] Jiang Yunyao;Liu Nan;Hu Xiaomei;Hou Jincai;Liu Jianxun(Institute for Chinese Materia Medica,School of Pharmaceutical Sciences,Tsinghua University,Beijing 100084,China;Beijing Increasepharm Safety and Efficacy Co.,Ltd,Beijing 102206,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Jing-Jin-Ji Joint Innovation Pharmaceutical(Beijing)Co.,Ltd.,Beijing 100083,China)

机构地区：[1]清华大学药学院中药研究院,北京100084 [2]北京盈科瑞药物安全有效性研究有限公司,北京102206 [3]中国中医科学院西苑医院,北京100091 [4]京津冀联创药物研究(北京)有限公司,北京100083

出　　处：《世界科学技术-中医药现代化》2020年第9期3142-3149,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家科学技术部国家重点基础研究发展计划(973计划)项目(2015CB554405):基于病证结合的气血相关理论研究,负责人:刘建勋。

摘　　要：目的采用网络药理学方法,探索八珍汤治疗原发免疫性血小板减少症(Primary immune thrombocytopenia,ITP)的作用机制。方法通过中药系统药理学分析平台(TCMSP)、Drugbank数据库和文献检索方式获得八珍汤8味中药的化学成分和作用靶点,以口服吸收利用度和药物相似性等条件进行筛选。通过OMIM数据库、TTD数据库、GAD数据库和GeneCards数据库查找ITP的相关靶点。进而利用Cytoscape3.7.1软件构建化学成分-靶点网络、疾病-靶点网络、成分靶点和疾病靶点交集网络,通过Cytoscape软件中ClueGO插件和DAVID在线分析工具对八珍汤治疗ITP的相关靶点进行基因本体(GO)功能富集分析和KEGG通路分析,探讨八珍汤治疗ITP的作用机制。结果共筛选得到159个八珍汤化学成分和146个靶点;获得306个ITP相关靶点;得到21个八珍汤治疗ITP的作用靶点;显著富集到114个生物过程条目和34条KEGG生物通路。结论八珍汤主要通过TNF信号通路、HIF-1信号通路、NOD样受体信号通路、VEGF信号通路和T细胞受体信号通路等发挥治疗ITP的作用,其中TNF信号通路可能是其关键机制。Objective To explore and investigate the mechanisms of Bazhen Decoction in the treatment of primary immune thrombocytopenia(ITP)by using network pharmacology approach.Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Drugbank database,and literature retrieval were used to obtain chemical components and targets of Bazhen Decoction combined with oral bioavailability and drug analysis to screen the components.OMIM database,TTD database,GAD database,and GeneCards database were used to search for the ITP-related targets.Then the compound-target network,ITP-target network,and interaction network of Bazhen Decoction and ITP-related targets were constructed by using Cytoscape 3.7.1 software.Gene ontology(GO)and KEGG pathway analysis were carried out by using the ClueGO plug-in of Cytoscape software and Database for Annotation,Visualization and Integrated Discovery(DAVID)to analyze the mechanisms of Bazhen Decoction in the treatment of ITP.Results In total,159 chemical components and 146 targets of Bazhen Decoction,and 306 ITP-related targets were obtained.In addition,21 candidate targets of Bazhen Decoction against ITP,114 terms of GO analysis,and 34 KEGG pathways were obtained.Conclusion Results suggested that TNF signaling pathway,HIF-1 signaling pathway,NOD-like receptor signaling pathway,VEGF signaling pathway,and T cell receptor signaling pathway were the key factors affecting the efficacy of Bazhen Decoction for treatment of ITP,in which TNF signaling pathway may be the key mechanism.

关 键 词：八珍汤 原发免疫性血小板减少症 网络药理学 作用机制 生物通路 

分 类 号：R285.6[医药卫生—中药学]