四君子汤对脾气虚证模型大鼠视网膜脱离复位后感光细胞凋亡的影响  

Effect of Sijunzi Decoction on Photoreceptor Apoptosis after Experimental Retinal Detachment Rats with Pi Qi Deficiency Syndrome

作　　者：任秀瑜 康紫薇 张妍春 屈超义 李亚芙 李京珂 REN Xiu-yu;KANG Zi-wei;ZHANG Yan-chun;QU Chao-yi;LI Ya-fu;LI Jing-ke(Department of Traditional Chinese Medicine、First Clinical Medical College,Shaanxi University of Traditional Chinese Medicine,Xi'an,712000;Department of Ophthalmology,Xi'an People's Hospital(Xi'an Fourth Hospital),Xi'an,710000;Xi'an Institute of Fundus Diseases,Xi'an,710000)

机构地区：[1]陕西中医药大学第一临床医学院中医系,陕西712000 [2]陕西省西安市人民医院(西安市第四医院)眼科,两安710000 [3]陕西省西安市眼底病研究所,西安710000

出　　处：《中国中西医结合杂志》2021年第2期204-211,共8页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：陕西省自然科学基础研究计划面上项目(No.2015JM8408);陕西省中医管理局中医药科研课题面上项目(No.JCMS063);西安市科技计划重大项目(No.201805104YX12SF38-3);陕西省西安市第四医院院内科研孵化基金项目(No.FZ-26)。

摘　　要：目的观察四君子汤对脾气虚型大鼠视网膜脱离(RD)自动复位后感光细胞凋亡的影响。方法成年SD大鼠40只随机分为对照组(A组)、RD自动复位组(B组)、脾气虚RD自动复位组(C组)、四君子汤组(D组)。C、D组使用破气耗气加饥饱失常法制备脾气虚证动物模型;于实验开始后6周B、C、D组使用视网膜下注射0.1%透明质酸钠的手术方法制备RD自动复位模型;并分别于术后2、3周分批处死动物取材。记录大鼠进食量、体重及大鼠精神状态;测定大鼠血清D-木糖值、眼内液超氧化物歧化酶(T-SOD)、丙二醛(MDA)水平;眼球石蜡切片HE染色、原位末端标记(TUNEL)染色;Western Blot分析视网膜Caspase-3、SOD2、缺氧诱导因子-1α(HIF-1α)蛋白表达量变化。结果与A、B组比较,C、D组于术前体重及进食量、血清D-木糖值明显降低(P<0.05),C组上述症状持续至实验结束,D组于灌服四君子汤后症状改善,术后3周,与A组比较,血清D-木糖值差异无统计学意义(P>0.05);与C组比较,D组进食量在术后3周显著增加(P<0.05)。与B组比较,术后2、3周C组眼内液T-SOD活性、视网膜组织SOD2、HIF-1α显著降低,视网膜组织Caspase-3、感光细胞凋亡指数显著上升(P<0.05);术后3周MDA水平显著升高(P<0.05);与C组比较,D组术后2、3周眼内液T-SOD活性、视网膜SOD2、HIF-1α显著增高(P<0.05),Caspase-3显著降低(P<0.05);术后3周MDA水平、感光细胞凋亡指数显著降低(P<0.05)。结论脾气虚证通过加重RD复位后眼内氧化应激损伤加重视网膜感光细胞凋亡的发生,使用益气健脾经方四君子汤能有效提高眼内抗氧化能力,减少脾气虚证大鼠RD复位后感光细胞凋亡,发挥神经保护作用。Objective To observe the effect of Sijunzi Decoction(SJZD)on photoreceptor apoptosis after experimental retinal detachment(RD)rats with Pi qi deficiency syndrome(PQDS).MethodsForty adult Spregue-Dawley rats were randomly divided into control group(Group A),RD automatical reattachment group(Group B),RD automatical reattachment with PQDS group(Group C),and SJZD treatment group(Group D).The PQDS model was established by dietary disorder,medicine-induced Pi-qi consumption in Group C and D.At week 6 after starting the experiment,the model of RD automatic reattachment was prepared by sub-retinal injecting 0.1%sodium hyaluronate to rats in Group B,C,and D.Animals were sacrificed by batches at week 2 and 3 after operation respectively.Food intake,body weight,and mental states of rats were recorded.The contents of D-xylose in serum,total superoxide dismutase(T-SOD)activities and malondialdehyde(MDA)contents in humor aqueous were measured.The retina were paraffin sliced by HE staining and TUNEL staining.Expressions of Caspase-3,superoxide dismutase 2(SOD2),and hypoxia-inducible factor-1α(HIF-1α)were analyzed by Western Blot.Results Pre-operative body weight,food intake,and the serum D-xylose level were significantly lower in Group C and D,as compared with those in Group A and B(P<0.05).In Group C,the above symptoms lasted to the end of the experiment.Rats′symptoms were improved in Group D after taking SJZD.There was no difference in serum D-xylose level between Group D and Group A at week 3 after operation(P>0.05).Compared with Group C,food intake in Group D significantly increased(P<0.05).Compared with Group B,the T-SOD activity in humor aqueous,the expressions of SOD2 and HIF-1αin retinal tissues decreased significantly,the expression of Caspase-3 in retinal tissues and photoreceptor cell apoptotic index significantly increased in Group C at post-operative week 2 and 3.And the MDA activity significantly increased at post-operative week 3(P<0.05).Compared with Group C,the T-SOD activity in humor aqueous,the expressio

关 键 词：四君子汤 脾气虚证 视网膜脱离自动复位 感光细胞凋亡 氧化应激 

分 类 号：R285.5[医药卫生—中药学]