复方青黛胶囊对银屑病微环境下HaCaT细胞分泌细胞因子的影响  

Effect of Compound Qingdai Capsule on Cytokines Secreted by HaCaT Cells Under Microenvironment of Psoriasis

作　　者：郑雯[1] 李刚刚 刘清民 王红 许美凤[1] 马慧群[1] ZHENG Wen;LI Ganggang;LIU Qingmin;WANG Hong;XU Meifeng;MA Huiqun(Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)

机构地区：[1]西安交通大学第二附属医院,陕西西安710004

出　　处：《中国皮肤性病学杂志》2021年第3期261-265,共5页The Chinese Journal of Dermatovenereology

基　　金：陕西省重点研发计划高校联合项目(2020GXLH-Y-008)。

摘　　要：目的探讨复方青黛胶囊在银屑病微环境下对HaCaT细胞IL-2、IL-6、IL-8、IL-18、IFN-γ表达水平的影响,初步阐明复方青黛胶囊治疗银屑病的可能机制。方法在银屑病微环境下观察复方青黛胶囊对HaCaT细胞IL-2、IL-6、IL-8、IL-18、IFN-γ表达水平的影响,并设空白对照和阿维A阳性对照。将HaCaT细胞分为4组,对照组(C组)不加任何干预;模型组(M组):采用M5(IL-1a、IL-17A、IL-22、抑癌蛋白M和TNF-α)模拟银屑病炎性微环境造模;复方青黛胶囊组(MF组)在模型组基础上加10%复方青黛胶囊培养72 h;阿维A组(MA组)在模型组基础上+10μmol/L阿维A培养72 h。采用ELISA方法检查各组细胞培养上清中IL-2、IL-6、IL-8、IL-18、IFN-γ的水平。结果M组与C组相比,IL-6、IL-8、IL-2、IFN-γ、IL-18水平显著升高,差异均有统计学意义(P均<0.05);MF组与M组相比IL-6、IL-8、IL-2、IFN-γ、IL-18水平显著降低,差异均有统计学意义(P均<0.05);MA组与M组相比IL-6、IL-2、IFN-γ、IL-18水平显著降低,差异均有统计学意义(P均<0.05),两组IL-8水平比较差异无统计学意义(P>0.05);MF组与MA组比较IL-6、IL-8、IFN-γ水平差异均有统计学意义(P均<0.05),两组IL-2、IL-18水平差异无统计学意义(P均>0.05)。结论复方青黛胶囊可能通过降低IL-6、IL-8、IL-2、IFN-γ、IL-18等银屑病相关炎性因子的水平而发挥治疗银屑病的作用。Objective To investigate the effect of compound Qingdai capsule on the expression levels of IL-2,IL-6,IL-8,IL-18 and IFN-γin HaCaT cells under the microenvironment of psoriasis,and to preliminarily clarify the possible mechanism of compound Qingdai capsule in the treatment of psoriasis.Methods Cultured HaCaT cells were divided into 4 groups.The control group(C)was treated without any intervention.Model group(M)were cultured with IL-1A,IL-17A,IL-22,tumor suppressor protein M and TNF-γto simulate the inflammatory microenvironment of psoriasis.Compound Qingdai capsule group(MF)was cultured with 10%compound Qingdai capsule for 72 h on the basis of model group.Acitretin group(MA)was cultured for 72 h with 10 mol/L acitretin on the basis of model group.The levels of IL-2,IL-6,IL-8,IL-18 and IFN-γin the supernatant of cell culture in each group were examined by ELISA.Results The levels of IL-6,IL-8,IL-2,IFN-γand IL-18 in M group were significantly higher than those in C group,and the differences were statistically significant(all P<0.05).The levels of IL-6,IL-8,IL-2,IFN-γand IL-18 in MF group were significantly lower than those in M group,and the differences were statistically significant(all P<0.05).The levels of IL-6,IL-2,IFN-γand IL-18 in MA group were significantly lower than those in M group with statistically significant differences(all P<0.05),while the levels of IL-8 in MA group and M group were not significantly different(P>0.05).There were statistically significant differences in IL-6,IL-8 and IFN-γlevels between MF and MA group(all P<0.05),while no statistically significant differences in IL-2 and IL-18 levels between MF and MA group(all P>0.05).Conclusion Compound Qingdai capsule may play a role in the treatment of psoriasis by reducing psoria-related inflammatory factors such as IL-6,IL-8,IL-2,IFN-γand IL-18.

关 键 词：复方青黛胶囊 银屑病 HACAT细胞 IL-6 IL-8 IL-2 IFN-γ IL-18 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]