张家口市性接触人群HIV-1遗传多样性与耐药分析  

HIV-1 Genetic Diversity and Drug Resistance in the Population Infected with Sexual Contact in Zhangjiakou

作　　者：刘琪琛[1] 冀永娟 马琳[3] 李岩[3] 尹少禹[1] 卢志红 赵浩文 王莹莹[3] 路新利[3] LIU Qichen;JI Yongjuan;MA Lin;LI Yan;YIN Shaoyu;LU Zhihong;ZHAO Haowen;WANG Yingying;LU Xinli(Zhangjiakou Center for Disease Control and Prevention,Zhangjiakou 075000,China;Health Commission of Zhangjiakou,Zhangjiakou 075000,China;Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021,China;The Fifth Hospital of Zhangjiakou,Zhangjiakou 075000,China)

机构地区：[1]张家口市疾病预防控制中心,河北张家口075000 [2]张家口市卫生健康委,河北张家口075000 [3]河北省疾病预防控制中心,河北石家庄050021 [4]张家口市第五医院,河北张家口075000

出　　处：《中国皮肤性病学杂志》2021年第3期288-292,共5页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨张家口市HIV-1遗传基因多样性和原发耐药基因突变情况。方法使用In-house方法扩增HIV-1蛋白酶和逆转录酶编码区基因序列,通过系统进化分析和耐药数据库比对确定HIV-1基因型和基因突变情况。结果张家口市未治疗人群中共有5种HIV-1基因亚型毒株在流行,其中CRF01_AE是最主要流行毒株,占到45.00%(9/20),其次是CRF07_BC(40.00%,8/20)。其他亚型包括B(5.00%,1/20)、CRF68_01B(5.00%,1/20)和CRF65_cpx(5.00%,1/20)。CRF68_01B是河北省首次发现的基因型。HIV-1原发耐药发生率为20.00%(4/20)。没有发现NRTI耐药基因突变。结论张家口市未接受抗病毒治疗人群中HIV-1遗传基因多样化,耐药基因突变水平较高,成簇传播,加强HIV-1耐药监测和预防控制措施研究已成当务之急。Objective To investigate HIV-1 genetic diversity and primary drug resistance.Methods In protease and reverse transcriptase gene coding region,HIV-1 gene sequences were amplified using in-house method,and HIV-1 genotypes and drug resistance mutations were analyzed by phylogenetic analysis and comparison of drug resistance database.Results Five genotypes were found in the untreated population in Zhangjiakou,Hebei.Of them,CRF01_AE were the most frequent,accounting for 45.00%(9/20),followed by CRF07_BC(40.00%,8/20).Other genotypes included B(5.00%,1/20),CRF68_01B(5.00%,1/20)and CRF65_cpx(5.00%,1/20).CRF68_01B was first found in Hebei.In this study,a total of four HIV-1 infected people occurred primary resistance mutations,with a total resistance rate of 20.00%(4/20).No NRTI mutation was found.Conclusion The resistance rate among naive HIV-infected persons in Zhangjiakou was higher,and HIV-1 resistant strains were circulating in clusters.Therefore,it was critical for us to strengthen HIV-1 resistance monitoring and prevention.

关 键 词：HIV-1 亚型 耐药突变 性接触传播 

分 类 号：R512.91[医药卫生—内科学]