化学发光法和TPPA试验检测妇幼人群梅毒螺旋体抗体假阳性对比分析  

Comparative Analysis of Chemoluminescence and TPPA Test for False-Positive of Treponema Pallidum Antibody in Women and Children

作　　者：郑淑华[1] 黄瑞玉[1] 卢春敏[1] 穆小萍[1] ZHENG Shuhua;HUANG Ruiyu;LU Chunmin;MU Xiaoping(Clinial Laboratory of Guangdong Women and Children Hospital,Guangdong 511400,China)

机构地区：[1]广东省妇幼保健院检验科,广东广州511400

出　　处：《中国皮肤性病学杂志》2021年第3期349-352,共4页The Chinese Journal of Dermatovenereology

摘　　要：目的分析2017-2019年本院妇幼人群梅毒螺旋体抗体化学发光法(CMIA)筛查阳性标本及其凝集试验(TPPA)复检的结果,评价两种方法的符合率,为选择筛查方法提供依据。方法回顾性分析梅毒CMIA的筛查结果,以及筛查阳性标本同时采用TPPA复检的结果838例,统计分析CMIA与TPPA结果的符合率,计算CMIA阳性而TPPA阴性结果的比率,分析结果不一致的人群分布。结果CMIA与TPPA总符合率为74.49%,当S/CO值>9.0时,两者符合率达100%,CMIA阳性而TPPA阴性结果比率为25.06%,其中S/CO值在>1.0~≤3.0之间的结果不一致比率为81.31%,所有结果不一致的人群中<5岁儿童占41.90%,育龄女性占58.10%,妊娠状态比例较高。结论梅毒CMIA法灵敏度高,阳性筛查结果S/CO值<9.0时需用TPPA法复检,妊娠状态容易干扰检测结果,需谨慎发出,避免实施不必要的母婴传播干预措施。Objective To analyze the positive samples of Treponema pallidum antibody with chemiluminescence(CMIA)and agglutination test(TPPA)for women and children in our hospital from 2017 to 2019,evaluate the coincidence rate of the two methods.Methods A retrospective analysis of 838 cases of syphilis CMIA screening results and TPPA retest results.The ratio of CMIA positive and TPPA negative results was calculated.And the distribution of people with inconsistent results was analyzed.Results The total coincidence rate of CMIA and TPPA was 74.49%.When the S/CO value was greater than 9.0,the coincidence rate of the two methods was 100%.The ratio of CMIA positive to TPPA negative was 25.06%.The rate of inconsistency among the results was 81.31%.Among the population with inconsistent results,children younger than 5 years accounted for 41.90%,women of childbearing age accounted for 58.10%,and the proportion of pregnancy status was higher.Conclusion Syphilis CMIA method has high sensitivity.When the positive screening result S/CO value is less than 9.0,it needs to be retested by TPPA method.The pregnancy status is a interference factors.It needs to be read the results carefully,to avoid implementing unnecessary mother-to-child transmission interventions.

关 键 词：梅毒 化学发光法 TPPA试验 妇幼人群 假阳性 

分 类 号：R446.5[医药卫生—诊断学] R759.1[医药卫生—临床医学]