T细胞内抗原1在乳腺癌中的甲基化状态及与乳腺癌多层螺旋CT征象相关性研究  

Research of correlation between methylation of TIA1 in breast cancer and multi-slice spiral CT signs

作　　者：王录兵[1] 黄勇[1] 符惠宏 雷星[1] 陈运奎 徐程华 Wang Lubing;Huang Yong;Fu Huihong;Lei Xing;Chen Yunkui;Xu Chenghud(Department of Radiology,the First People's Hospital of Taizhou City,Zhejiang 318020,China;Department of Neurology,Taizhou First People's Hospital,Zhejiang 318020,China)

机构地区：[1]浙江省台州市第一人民医院放射科,318020 [2]浙江省台州市第一人民医院神经内科,318020

出　　处：《中华内分泌外科杂志》2021年第1期85-88,共4页Chinese Journal of Endocrine Surgery

基　　金：浙江省基础公益研究计划项目(LGF19H090001)。

摘　　要：目的探究T细胞内抗原1(T-cell intracellular antigen 1,TIA1)在乳腺癌组织中的表达和TIA1启动子区甲基化状态,并分析乳腺癌多层螺旋CT征象与TIA1甲基化状态之间的关系。方法选取2019年2月至2020年3月浙江省台州市第一人民医院收治的50例乳腺癌患者,荧光定量PCR法检测所有患者肿瘤组织和癌旁组织TIA1表达水平。通过生物信息学软件MethPrimer预测TIA1启动子区域发现存在cPG岛。采用甲基化特异性聚合酶链反应(Methylmion Specific PCR,MSP)检测肿瘤组织和癌旁组织中TIA1甲基化状态,采用多层螺旋CT对患者进行检查,观察肿块大小、形态、钙化区域、淋巴结转移及边缘,分析TIA1甲基化状态与CT征象之间的相关性。结果乳腺癌组织TIA1表达水平低于癌旁组织(0.50±0.12 vs 0.95±0.10,P=0.00),但TIA1基因启动子甲基化率高于癌旁组织(64% vs 42%,χ2=4.86,P<0.05)。乳腺癌患者TIA1基因启动子甲基化率在肿块形态和有无钙化方面差异无统计学意义(P>0.05)。肿块直径≥2 cm患者TIA1基因启动子甲基化率显著高于肿块直径<2 cm患者(78.57% vs 45.45%,P<0.05),且淋巴结转移患者TIA1基因启动子甲基化率显著高于无淋巴结转移患者(79.17% vs 50.00%,P<0.05)。肿块边缘有毛刺的患者TIA1基因启动子甲基化率显著高于无毛刺的患者(80.77% vs 45.83%,P<0.05)。结论乳腺癌CT显像与TIA1基因启动子甲基化率之间存在相关性,这可为乳腺癌恶变程度和预后的判断提供依据。Objective To detect the expression and methylation of TIA1 in breast cancer and to study its correlation with multi-slice spiral CT signs.Methods 50 patients with breast cancer were collected from Feb.2019 to Mar.2020.The expressi on levels of TIAl in breast cancer tissues and in peri tumoral tissues were estimated by quantitative real-time polymerase chain reaction.Bioinformatics software MethPrimer was used for pre-dieting TIAl promotor and confirmed the existenee of cPG island.Methylation-specific PCR(MSP)was performed to detect TIAl DNA promoter methylation.All patients were examined by multi-slice CT.CT images were analyzed through observing the tumor size,shape,calcification area,lymph node metastasis and margin.The correlation between CT signs and TIAl methylation status was further analyzed.Results The expression levels of TIAl in breast cancer tissues were lower than in peritumoral tissues(0.50±0.12,0.95±0.10,P=0.00),while TIAl DNA promoter methylation rate was higher than in peritumoral tissues(64%,42%,X^2=4.86,P<0.05).There were no significant differences in TIA1 DNA promoter methylation rate among patients with different tumor shape and micro calcifications.TIAl DNA promoter methylation rate in patients with mass diametercm were significantly higher than those in patients with mass diameter<2 cm(78.57%,45.45%,P<0.05),and TIAl DNA promoter methylation rate in patients with lymph node metastasis was higher than those without lymph node metastasis(79.17%,50%,P<0.05).TIAl DNA promoter methylation rate in patients with burr at edge of mass was higher than those wihout burr at edge of mass(80.77%,45.83%,P<0.05).Conclusion There is a correlation between CT imaging signs and TIA1 DNA promoter methylation rate in patients with breast cancer,which can provide more reference for the judgment of malignant degree and prognosis of patients with breast cancer.

关 键 词：多层螺旋CT 乳腺癌 T细胞内抗原1 甲基化 

分 类 号：R655.8[医药卫生—外科学] R737.9[医药卫生—临床医学]