PLGA静电纺丝纳米纤维结合诱导培养基和BMP4诱导鼠胚胎干细胞向牙上皮分化  

PLGA electrospinning nanofier combined with induction medium and BMP4 induces mouse embryonic stem cells differentiate into dentogenic epithelial cells

作　　者：张彦聪 窦林波[2] 张风河[1] ZHANG Yancong;DOU Linbo;ZHANG Fenghe(School of Stomatology,Shandong University,250000,China;Dezhou People's Hospital,Shandong Province)

机构地区：[1]山东大学口腔医学院,250000 [2]德州市人民医院

出　　处：《实用口腔医学杂志》2021年第1期19-23,共5页Journal of Practical Stomatology

基　　金：山东省医药卫生科技发展计划项目(编号:2017WS736,2019WS020)。

摘　　要：目的:检测聚羟基乙酸聚乳酸共聚物(PLGA)纳米支架材料结合诱导培养基和BMP4对胚胎鼠干细胞(mES)向牙上皮细胞分化的影响。方法:用PLGA作为基底材料,通过静电纺丝技术构建纳米纤维支架材料,结合不同时间点BMP4的干预,将鼠胚胎干细胞向牙上皮细胞诱导,构建mES向牙上皮分化的诱导体系,并进一步筛选出mES细胞向牙上皮分化的最佳诱导方案。结果:在培养初期抑制BMP448 h,然后在培养液中短时间(48 h)或持续加入外源性BMP4,48 h后可检测到成釉蛋白(AMBN)阳性的细胞。结论:PLGA纳米纤维表面结合诱导培养基和BMP4可将mES细胞诱导为牙上皮细胞。Objective:To study the effects of Poly lactic-co-glycolic acid(PLGA)nanofiber scaffold with induction medium and BMP4 on the differentiation of mouse embryogenetic stem(mES)cells into dental epithelium cells.Methods:PLGA was used as the basal material for the preparation of nanofiber scaffold by electrostatic spinning technology in combination with induction medium and BMP4.mES cells were culture on the nanofiber scaffold for different time points of intervention with induction medium and BMP4 for mES cells to differentiate into dental epithelium.Furthermore,the optimal induction scheme of mES cells differentiation was selected.Results:The first 48 h inhibition and then a short period of time(48 h)or continuous addition of exogenous BMP4 in the medium showed that ameloblastin(AMBN)positive cells could be detected by adding BMP4 in the medium for 48 h.Conclusion:The surface of PLGA nanofibers combined with induction medium and BMP4 can induce mES cells to differentiate into dental epithelial cells.

关 键 词：鼠胚胎干细胞 牙上皮细胞 BMP4 支架材料 

分 类 号：R318.08[医药卫生—生物医学工程]