大葱叶际生防细菌筛选及对紫斑病防效研究  

Screening for biocontrol bacteria against Alternaria porri from phyllosphere of welsh onion

作　　者：孙婷婷[1] 赵松宇 王新法[1] 秦明一[2] 张超[2] 田雪亮[1] SUN Tingting;ZHAO Songyu;WANG Xinfa;QIN Mingyi;ZHANG Chao;TIAN Xueliang(Henan Engineering Research Center of Biological Pesticide&Fertilizer Development and Synergistic Application,Henan Institute of Science and Technology,Xinxiang 453003,China;Yangtze Normal university,Chongqing 408100,China)

机构地区：[1]河南科技学院河南省生物药肥研发与协同应用工程研究中心,河南新乡453003 [2]长江师范学院,重庆408100

出　　处：《河南科技学院学报（自然科学版）》2021年第1期35-40,共6页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：国家重点研发计划项目(2018YFD0201200)。

摘　　要：为了挖掘大葱紫斑病生防资源,研究采用培养法从大葱叶际分离细菌,然后用对峙培养法筛选大葱紫斑病菌生防细菌.结果表明:大葱叶际细菌群落中嗜甲基单胞菌、鞘氨醇单胞菌属、微杆菌属、假单胞菌属于优势细菌类群.从中筛选出1株对大葱紫斑病菌具有较好拮抗活性的菌株X85,经形态特征和16S rDNA序列鉴定为亚麻假单胞菌.田间试验表明该菌对大葱紫斑病的防效为63.1%.该研究为后续开发大葱紫斑病生物防治奠定基础.To develop biocontrol resource against Alternaria porri,bacteria were isolated from phyllosphere of welsh onion by cultured method and screened for biocontrol bacteria using confrontation culture.The result showed that Methylobacterium,Sphingomonas,Microbacterium and Pseudomonas were the dominant groups in the community of phyllosphere.One strain bacterium with high activity of against A.porri was obtained from the phyllosphere bacteria group.According to morphological characteristics and 16S rDNA sequence,the bacterium was identified as Pseudomonas lini.The control efficiency of P.lini against Alternaria leaf spot was 63.1%by field trial.This will lay the foundation for the further development of biological control of the disease.

关 键 词：叶际细菌 大葱紫斑病 亚麻假单胞菌 

分 类 号：S432[农业科学—植物病理学]