转基因大豆CP4-EPSPS蛋白的降解规律研究  

Research on degradation of CP4-EPSPS protein from transgenic soybean

作　　者：李允静[1,2,3] 万丹凤 刘标 肖芳 李晓飞 武玉花[1,2,3] 李俊 高鸿飞[1,2,3] 沈文静 李均[1,2,3] 朱莉 吴刚 LI Yun-jing;WAN Dan-feng;LIU Biao;XIAO Fang;LI Xiao-fei;WU Yu-hua;LI Jun;GAO Hong-fei;SHENG Wen-jing;LI Jun;ZHU Li;WU Gang(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Key Labora-tory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,Chi-na;Supervision and Test Center(Wuhan)for Environmental Safety of Genetically Modified Plants,Ministry of Agri-culture and Rural Affairs,Wuhan 430062,China;Nanjing Institute of Environmental Sciences,Ministry of Ecology and Evironment,Nanjing 210042,China)

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [3]农业农村部转基因植物环境安全监督检验测试中心(武汉),湖北武汉430062 [4]生态环境部南京环境科学研究所,江苏南京210042

出　　处：《中国油料作物学报》2021年第1期124-130,共7页Chinese Journal of Oil Crop Sciences

基　　金：转基因生物新品种重大专项(2016ZX08012-005);中国农业科学院科技创新工程项目(2020-2060302-049-091)。

摘　　要：随着转基因技术发展和应用,转基因外源基因表达蛋白在环境中富集和降解情况成为公众关注的问题,外源蛋白在环境中的检测和监测成为转基因研究和监管的一个方向。本研究利用模拟自然降解和蛋白酶K两种方式,处理转基因大豆种子(5%GTS 40-3-2),采用Western blot、双抗夹心快速检测试纸条和酶联免疫吸附测定(ELISA)等手段检测CP4-EPSPS蛋白降解情况,结果发现在模拟自然降解条件下,CP4-EPSPS蛋白呈现逐渐被降解的趋势,且在17周被彻底降解;蛋白酶K处理大豆匀浆情况下,9小时CP4-EPSPS蛋白被快速降解。该研究探索了CP4-EPSPS蛋白在自然环境下的降解规律,为寻求一种快速、安全、有效降解转基因CP4-EPSPS蛋白研究提供参考。With the development and application of transgenic technology,enrichment and degradation of transgnic foreign protein in environment has become a public concern.Detection and monitoring of exogenous protein in the environment has become a direction of genetically modified organism research and supervision.In this study,transgenic soybean(5%GTS40-3-2)seeds containing CP4-EPSPS protein were treated by simulated natural degradation and protease K.Western blot,lateral flow test strip and enzyme linked immunosorbent assay(ELISA)were selected to detect the presence of CP4-EPSPS.The results showed that CP4-EPSPS was gradually degraded under simulated natural degradation conditions,and completely degraded in about 17 weeks.In the condition of protease K treatment,CP4-EPSPS was rapidly degraded in 9 hours.This study explored the degradation rule of CP4-EPSPS in natural environment.And it provided new ideas for the future research of rapid,safe and effective degradation of CP4-EPSPS.

关 键 词：转基因大豆 CP4-EPSPS 蛋白质 降解 

分 类 号：S565.1[农业科学—作物学]