水貂阿留申病毒诱导猫肾细胞(CrFK)凋亡  

Apoptosis induced by Aleutian mink disease virus in CrFK cells

作　　者：栾美慧 葛桂阳 宫庆龙[2] 徐宁 郜艳雪 时坤[1] 李健明[1] 刘艺 李东丽 孙志博 冷雪[1] 杜锐[3] LUAN Meihui;GE Guiyang;GONG Qinglong;XU Ning;GAO Yanxue;SHI Kun;LI Jianming;LIU Yi;LI Dongli;SUN Zhibo;LENG Xue;DU Rui(College of Chinese Medicine Materials,Jilin Agricultural University,Changchun 130118,China;College of Animal Science and Technology,J ilin Agricultural University,Changchun 130118,China;Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education of the People’s Republic of China,Laboratory of Production and Product Application of Sika Deer of Jilin Province,Jilin Agricultural Univercity,Changchun 130118,China)

机构地区：[1]吉林农业大学,中药材学院,吉林长春130118 [2]吉林农业大学,动物科学技术学院,吉林长春130118 [3]吉林农业大学,教育部动物生产及产品质量安全重点实验室/吉林省梅花鹿生产与产品应用研究室,吉林长春130118

出　　处：《中国兽医学报》2021年第1期56-61,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金面上资助项目(31272565);吉林省科技发展计划资助项目(20190304004YY)。

摘　　要：为研究水貂阿留申病毒(AMDV)在体外诱导猫肾细胞(CrFK)凋亡情况,用AMDV-G株感染CrFK细胞,采用CCK-8法检测CrFK细胞感染后的细胞存活率,用AO/EB染色法检测CrFK细胞核的形态变化,用Annexin V-FITC/PI双染流式细胞术检测细胞凋亡率,并通过分光光度计法检测细胞凋亡执行分子Caspase-3活性。结果显示,AMDV-G株感染可导致CrFK细胞增殖率下降,产生明显的形态学凋亡特征;流式细胞术检测结果显示,AMDV-G株感染可引起CrFK细胞凋亡并随着感染时间的延长凋亡率增加,同时Caspase-3活性显著升高。上述结果表明,AMDV-G株在体外能够诱导CrFK细胞凋亡,为进一步研究AMDV致病机理奠定基础。In order to study the apoptosis of CrFK cells induced by Aleutian mink disease virus(AMDV)in vitro,CrFK cells were infected with the AMDV-G strain.The survival rate of CrFK cells was detected by the CCK-8 assay.The morphological changes of nuclei of CrFK cells were detected by AO/EB staining.Apoptosis rate was determined by Annexin V-FITC/PI double staining flow cytometry assay.The activity of Caspase-3 was detected by spectrophotometer.The results showed that the AMDV-G strain could reduce the proliferation rate of CrFK cells and result in obvious morphological characteristics of apoptosis.The apoptosis rate and the activity of Caspase-3 increased with the extension of AMDV infection time.The above results showed that AMDV-G strain could induce the apoptosis of CrFK cells in vitro.The study lay the foundation for further research on the pathogenesis of AMDV.

关 键 词：水貂阿留申病毒 猫肾细胞 细胞凋亡 

分 类 号：S852.65[农业科学—基础兽医学]