羊源肺炎克雷伯菌的分离鉴定及毒力基因检测  

Isolation,identification and virulence gene detection of Klebsiella pneumoniae from sheep

作　　者：范培超 史量全 温树波 霍晓伟[1] 薛江东[1] 马德慧[1] FAN Peichao;SHI Liangquan;WEN Shubo;HUO Xiaowei;XUE Jiangdong;MA Dehui(College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao,Inner Mongolia 028000,China)

机构地区：[1]内蒙古民族大学,动物科学技术学院,内蒙古通辽028000

出　　处：《中国兽医学报》2021年第1期96-101,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31660726);内蒙古自治区自然科学基金资助项目(2018LH03016,2017MS0319);内蒙古自治区肉牛疾病防控工程技术研究中心开放课题资助项目(MDK2018021)。

摘　　要：为了调查内蒙古通辽市某羊场发病羊死亡的原因,对疑似致病菌进行分离、鉴定及部分生物学特性研究。对病死羊进行剖检,无菌条件下采集心脏、肝脏、脾脏、肺脏等组织脏器进行细菌分离培养;对分离菌进行小鼠致病性试验、细菌生化检测、16S rDNA基因扩增及同源性比对分析和药物纸片扩散法敏感性试验。结果显示,分离菌株符合肺炎克雷伯菌生化特性;16S rDNA序列与GenBank中已登录的肺炎克雷伯杆菌序列同源性为99.59%;小鼠致病性试验结果表明分离株有较强的致死性;分离株携带多种毒力基因致病性较强。药敏试验结果表明分离株对诺氟沙星、环丙沙星、氧氟沙星、多西环素等药物高度敏感,对复方新诺明、卡那霉素等中度敏感,对庆大霉素、头孢哌酮、头孢氨苄等耐药。结果表明,分离株为肺炎克雷伯菌,是该羊场发病羊死亡的致病菌。To investigate the cause of death of sheep in a sheep farm in Tongliao City,dead sheep was dissected to collect the heart,liver,spleen,lung and some other tissues under sterile condition.Bacterial isolation,culture,pathogenicity in mice,bacterial biochemical detection,16 S rRNA gene amplification,homology comparison analysis and drug sensitivity test were conducted.The results showed that the biochemical characteristics of the strain isolated from lungs were similar to that of the Klebsiella pneumoniae.The 16 S rDNA gene sequence of the isolate was 99.59%homology with the sequence of a Klebsiella pneumoniae strain available in GenBank.What?s more,the isolated strain contained a variety of toxin genes which was highly pathogenic to mice.In conclusion,a strain of Klebsiella pneumoniae containing many toxin genes was successfully isolated and the death of sheep in Tongliao City was suspected to be related to this bacterium.

关 键 词：羊源肺炎克雷伯菌 分离鉴定 16S rDNA 毒力基因 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]