肾阳虚型慢性再障患者CD3+CD19-和CD3+CD25+T细胞与血小板的多重线性回归分析  

Multiple Linear Regression Analysis of CD3_+ CD19_- and CD3_+ CD25_+ T Cells and Platelets in Kidney Yang Deficiency Patients with Chronic Aplastic Anemia

作　　者：丁宇斌 唐旭东[2] Ding Yubin;Tang Xudong(Graduate School of China Academy of Chinese Medical Sciences,Beijing 100700,China;Department,of Hematology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院研究生院,北京100700 [2]中国中医科学院西苑医院血液科,北京100091

出　　处：《世界科学技术-中医药现代化》2020年第11期3882-3886,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家中医药管理局中医药行业科研专项分任务(201507001-13):补肾生血和补气养血法对照治疗再生障碍性贫血增加疗效及降低复发率的随机对照研究,负责人:唐旭东;国家自然科学基金面上项目(81673819):TRIF信号通路在青黄散不同配比治疗骨髓增生异常综合征的作用和机制研究;负责人:唐旭东。

摘　　要：目的建立多重线性回归模型分析肾阳虚型慢性再生障碍性贫血(Chronic aplastic anemia,CAA)患者的总T细胞(CD3+CD19-)、活化T细胞(CD3+CD25+)与血小板(PLT)的线性关系并探讨肾阳虚型CAA患者PLT数量在治疗转归中的预测因素。方法纳入22例肾阳虚型CAA患者,收集外周血中CD3+CD19-T细胞和CD3+CD25+T细胞占淋巴细胞的比例和PLT共3个参数并进行线性回归分析。结果因学生化删除残差(SDR_(1))超过3倍标准差的离群值以及强杠杆点(LEV_(1))> 0.5者各有1条观测值,对这2条观测值予以删除。回归模型具有统计学意义F(2,17)=19.479(P <0.001),R^(2)=0.696,调整R2=0.660。纳入模型的2个自变量(CD3+CD19-)和(CD3+CD25+)对PLT的影响均有统计学意义(P <0.05),并得到回归方程:PLT=391.585-5.104×(CD3+CD19-)+23.851×(CD3+CD25+)。结论外周血淋巴细胞中CD3+CD19-T细胞的比例和CD3+CD25+T细胞的比例可用于有效地估计和预测肾阳虚型CAA患者在未来临床转归中PLT的值及其变化趋势,有助于临床医师及时判断和评估CAA患者在临床结局中的出血风险及出血症状对患者生存质量的影响,具有较好的预测价值和临床实践意义。Objective To establish a multiple linear regression model to analyze the linear relationship between total T cells(CD3+CD19-), activated T cells(CD3+CD25+) and platelets(PLT) in kidney yang deficiency patients with chronic aplastic anemia(CAA) and to explore the predictive factors of PLT number in the treatment outcome of CAA in kidney yang deficiency patients. Methods 22 CAA patients with kidney yang deficiency were enrolled. 3 parameters including CD3+CD19-T cell ratio in peripheral blood, CD3+CD25+T cell to lymphocyte ratio and PLT were collected and analyzed by linear regression. Results Due to Studentized Deleted Residuals(SDR1) exceeding 3 times the standard deviation and those with strong leverage points(LEV1) > 0.5, these 2 observations were removed. The regression model had statistical significance F(2, 17) = 19.479(P < 0.001), R^(2)= 0.696, and adjusted R2= 0.660. The effects of the 2 independent variables included in the model on PLT were statistically significant(P < 0.05), and the regression equation was obtained: PLT = 391.585-5.104 ×(CD3+CD19-) + 23.851 ×(CD3+CD25+). Conclusion The proportion of CD3+CD19-T cells and CD3+CD25+T cells in peripheral blood lymphocytes can be used to effectively estimate and predict the PLT number and its trend in the future clinical outcome of CAA patients with kidney yang deficiency, which is helpful for clinicians to timely judge and evaluate the bleeding risk and the impact of bleeding symptoms on the quality of life of CAA patients in the clinical outcome and has better predictive value and clinical significance.

关 键 词：慢性再生障碍性贫血 免疫分型 血小板 多重线性回归 肾阳虚 

分 类 号：R256[医药卫生—中医内科学]