AQP2、HSP70在痛风湿热蕴结证模型中表达的意义  

Significance of AQP2 and HSP70 Expression in Gout Model with Damp-Heat Syndrome

作　　者：郭玉星[1,2] 朱珊莹 康佩芝[2] 汤智 易法银 邵先舫[3] 熊辉 刘永利[3] 毛果 Guo Yuxing;Zhu Shanying;Kang Peizhi;Tang Zhi;YI Fayin;Shao Xianfang;Xiong Hui;Liu Yongli;Mao guo(Hunan University of TCM,Changsha 410208,China;Affiliated Hospital of Hunan Institute of TCM,Changsha 410006,China;Chang de First Hospital of TCM,Chang de 415100,China;Xiangtan Hospital of Traditional Chinese Medicine,Xiangtan 411100,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南省中医药研究院附属医院,长沙410006 [3]常德市第一中医医院,常德415100 [4]湘潭市中医院,湘潭411100

出　　处：《世界科学技术-中医药现代化》2020年第11期3894-3899,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会面上项目(81574005):基于TLR4/NF-κB信号通路的蠲痹历节清方治疗痛风性关节炎机制研究,负责人:熊辉;湖南省中医药科研计划项目重点项目(201913):基于P2X7R/NLRP3途径探讨痛风清热颗粒治疗急性痛风性关节炎的效应机制,负责人:郭玉星;湖南省中医药研究院院级重点课题(201807):蠲痹历节清方抗高尿酸血症作用及与尿酸转运蛋白相关性研究,负责人:郭玉星。

摘　　要：目的采用多因素复合的造模方法,先建立湿热模型,采用改良Coderre[1]法痛风造模,以复制痛风湿热蕴结证病证结合动物模型[2],探明水通道蛋白-2(AQP2)、热休克蛋白70(HSP70)在痛风湿热蕴结证中表达的意义,为模型建立指标评估及临床药物疗效判定指标做进一步实验验证。方法实验对象为SPF级雄性SD大鼠,将40只大鼠按随机数字表分为4组,分别为正常对照组、痛风模型组、湿热模型组、痛风湿热模型组,每组10只。对各组分别造模(方法详见正文造模部分),造模期间观察大鼠外观、精神状态、食饮量、体质量变化等一般情况,测定受试踝关节炎症指数、造模72 h各组大鼠的步态情况、血尿酸、尿液AQP2、血浆HSP70等指标。结果4组模型尿液AQP2含量具有显著差异;与正常对照组比较,湿热模型组、痛风湿热模型组尿液AQP2明显升高(P<0.05;P<0.05);与痛风模型组对比,湿热模型组、痛风湿热模型组尿液AQP2明显升高(P<0.05;P<0.05)。四组模型尿液HSP70含量具有显著差异;与正常对照组比较,湿热模型组、痛风湿热模型组血浆HSP70明显升高(P<0.05;P<0.05);与痛风模型组对比,湿热模型组、痛风湿热模型组血浆HSP70明显升高(P<0.05;P<0.05)。结论通过多因素(饮食+气候环境+致病因子+局部用药+全身用药)复合造模方法可以复制出痛风湿热蕴结证病证结合模型,而且AQP2、HSP70两个指标在判断痛风湿热蕴结证型时有着重要作用,AQP2和HSP70含量变化可作为痛风湿热蕴结证模型建立成功与否的评价参考指标。Objective To establish a damp-heat model first based on a multi-factor modeling approach, and a modified Coderre method was used to model gout in order to replicate the the gout animal model with damp-heat syndrome, to explore the significance of the expression of aquaporin-2(AQP2) and heat shock protein70(HSP70) in the gout model with damp-heat syndrome, and to make further experimental validation for the evaluation of model establishment index and clinical drug efficacy determination index. Methods 40 healthy male SD rats were randomly divided into 4 groups,normal control group, gout model group, gout model with damp-heat syndrome group, damp-heat syndrome model group.Each group had 10 rats. Models were set up(the specific method was provided in full text) and the following general conditions were observed such as appearance, mental status, food and drink intake and weight changes. The inflammation index of the ankle joint, the gait condition of each group at 72 hours of modeling, blood uric acid, urine AQP2, plasma HSP70 and other indexes were measured. Results Urinary AQP2 levels in the 4 model groups were significantly different;Compared with the normal control group, urinary AQP2 was significantly higher in the gout model with damp-heat syndrome group and damp-heat syndrome model group(P < 0.05;P < 0.05);Urinary HSP70 levels in the 4 model groups were significantly different;Compared with the normal control group, plasma HSP70 was significantly higher in the gout model with damp-heat syndrome group and damp-heat syndrome model group(P < 0.05;P < 0.05);Compared with the gout model group, plasma HSP70 was significantly higher in the he gout model with damp-heat syndrome group and damp-heat syndrome model group(P < 0.05;P < 0.05). Conclusion Through the muiti factor diet and climate and pathogenic factor and topical and systemic medication combined modeling method can build the gout animal model with damp-heat syndrome. The AQP2 and the HSP70 index plays an important role in judging the painful rheumatism heat

关 键 词：痛风 湿热蕴结 复合模型 水通道蛋白-2 热休克蛋白70 

分 类 号：R259[医药卫生—中西医结合]