Design and implementation of the galvanometer scanning system for reflectance confocal and stimulated Raman scattering microscopy  

作　　者：Fangyu Wang Yuhao Yuan Qiang Sun Ming Dai Li Ai Fake Lu 王方雨;Yuhao Yuan;孙强;戴明;艾莉;Fake Lu(Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China;University of Chinese Academy of Sciences,Beijing 100049,China;Jilin Yataizhongke Medical Equipment Engineering Technology Research Institute Holding Co.,Ltd.,Changchun 130000,China;Department of Biomedical Engineering,Binghamton University,State University of New York,Binghamton,NY 13902,USA;Jilin Provincial Key Laboratory of Photoelectric Equipment and Instrument Advanced Manufacture Technology,Changchun 130033,China;Changchun UP Optotech Holding Co.,Ltd.,Changchun 130033,China)

机构地区：[1]Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Jilin Yataizhongke Medical Equipment Engineering Technology Research Institute Holding Co.,Ltd.,Changchun 130000,China [4]Department of Biomedical Engineering,Binghamton University,State University of New York,Binghamton,NY 13902,USA [5]Jilin Provincial Key Laboratory of Photoelectric Equipment and Instrument Advanced Manufacture Technology,Changchun 130033,China [6]Changchun UP Optotech Holding Co.,Ltd.,Changchun 130033,China

出　　处：《Chinese Optics Letters》2020年第12期44-48,共5页中国光学快报（英文版）

基　　金：the China Scholar・ship Council(No.201904910117);Jilin Province Talent Development Fund[2018]853 awarded to F.Wang。

摘　　要：We report on two strategies to design and implement the galvanometer-based laser-scanning mechanisms for the realization of reflectance confocal microscopy(RCM) and stimulated Raman scattering(SRS) microscopy systems. The RCM system uses a resonant galvanometer scanner driven by a home-built field-programmable gate array circuit with a novel dual-trigger mode and a home-built high-speed data acquisition card. The SRS system uses linear galvanometers with commercially available modules. We demonstrate video-rate high-resolution imaging at 11 frames per second of in vivo human skin with the RCM system and label-free biomolecular imaging of cancer cells with the SRS system. A comparison of the two strategies for controlling galvanometer scanners provides scientific and technical reference for future design and commercialization of various laser-scanning microscopes using galvanometers.

关 键 词：GALVANOMETER reflectance confocal microscopy stimulated Raman scattering:skin imaging cancer cells 

分 类 号：TH742[机械工程—光学工程]