分子印迹聚合物/多壁碳纳米管复合物电化学传感器对4-氨基水杨酸的检测研究  

A electrochemical sensor based on the composite of multi-walled carbon nanotubes and molecularly imprinted polymer for determination of 4-aminosalicylic acid

作　　者：李根根 牛丽婷 孙曼曼 林慧澍 张健夫 刘伟禄[2] LI Gengen;NIU Liting;SUN Manman;LIN Huishu;ZHANG Jianfu;LIU Weilu(School of Chemistry and Environmental Engineering,Changchun University of Science and Technology,Changchun 130022,China;School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 117004,China)

机构地区：[1]长春理工大学化学与环境工程学院,吉林长春130022 [2]沈阳药科大学药学院,辽宁本溪117004

出　　处：《沈阳药科大学学报》2020年第12期1084-1092,共9页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81503037);沈阳药科大学大学生创新创业训练计划项目(201810163025)。

摘　　要：目的制备基于分子印迹聚合物(MIP)/多壁碳纳米管(MWCNT)复合物的电化学传感器及高灵敏检测4-氨基水杨酸。方法以邻苯二胺为功能单体,4-氨基水杨酸为模板分子,在多壁碳纳米管修饰玻碳电极(GCE)上制备一层分子印迹聚合物膜,构建了分子印迹电化学传感器。实验采用扫描电子显微镜(SEM)和循环伏安法(CV)对电极材料进行了表征。优化了电极的制备条件。研究了分子印迹电化学传感器对模板分子及其结构类似物的选择性。结果在最佳实验条件下,该传感器对4-氨基水杨酸的线性响应范围为0.2~400μmol·L^(-1),检测限为0.024μmol·L^(-1),且表现出良好的选择性、稳定性以及重复性。此外,该传感器已被成功地用于尿样中4-氨基水杨酸的检测,加标回收率为92.4%~103.6%。结论所构建的电化学传感器可用于4-氨基水杨酸的检测研究。Objective A novel electrochemical sensor was developed based on molecularly imprinted polymer(MIP) and multiwalled carbon nanotube(MWCNT) to determine 4-aminosalicylic acid.Methods The MIP film was prepared by electropolymerization of o-phenylenediamine on a MWCNT modified electrode in the presence of 4-aminosalicylic as a template molecule.The MIP/MWCNT was characterized by scanning electron microscopy(SEM) and cyclic voltammetry(CV).The influence of the preparing conditions on the sensing performance was optimized.The selectivity of molecularly imprinted electrochemical sensors was investigated.Results Under the optimized condition,a wide linear range(0.2-400 μmol·L^(-1)) and a low detection limit(0.024 μmol·L^(-1)) were obtained.The sensor was successfully applied to detect 4-aminosalicylic in human urine sample with satisfied added standard recovery(92.4%-103.6%).Conclusion The electrochemical sensor can be used to detect 4-aminosalicylic acid.

关 键 词：4-氨基水杨酸 多壁碳纳米管 分子印迹聚合物 电化学传感器 

分 类 号：R917[医药卫生—药物分析学]