地衣芽孢杆菌对热应激公兔甲状腺和甲状旁腺组织的影响  

Effects of Bacillus licheniformis on thyroid and parathyroid gland tissue in male rabbits with heat stress

作　　者：韩昱 张立永[2] 张富梅[2] 冯宇飞 金圆圆 HAN Yu;ZHANG Liyong;ZHANG Fumei;FENG Yufei;JIN Yuanyuan(Laboratory Ani-mal Center,Hebei North University,Zhangjiakou,Hebei 075000,China;College of Science and Technology,Hebei North University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院实验动物中心,河北张家口075000 [2]河北北方学院动物科技学院,河北张家口075000

出　　处：《中国兽医学报》2021年第2期259-263,共5页Chinese Journal of Veterinary Science

基　　金：河北省科技计划资助项目(12226613,15226604D)。

摘　　要：将成年塞北兔公兔随机分成3组:常温组、热应激组(基础日粮)和地衣芽孢杆菌组(基础日粮+1000 mg/kg地衣芽孢杆菌),常温组温度为20~25℃,其他2个组人工模拟夏季温度。在试验期间测定血浆皮质醇含量。试验结束后测定甲状腺质量,计算甲状腺指数;观察光镜下甲状腺组织、透射电镜下甲状腺滤泡上皮细胞和甲状旁腺嗜酸性细胞变化。结果显示,地衣芽孢杆菌可极显著降低热应激公兔皮质醇含量,提高热应激15 d后公兔甲状腺质量和指数(P<0.01);明显改善甲状腺滤泡结构,膜结构较为清晰,降低细胞核萎缩和胞质内细胞器损伤;降低甲状旁腺嗜酸性细胞中线粒体空泡化程度。结果表明,在塞北兔公兔日粮中添加1000 mg/kg地衣芽孢杆菌可有效降低热应激公兔血浆皮质醇含量,缓解对甲状腺和甲状旁腺的损伤,对保证塞北兔公兔正常生殖机能具有重要作用。To investigate the mechanism of Bacillus licheniformis in relieving heat stress in male rabbits,adult male rabbits were randomly divided into 3 groups:normal temperature group,heat stress group(basic diet)and Bacillus licheniformis group(basic diet+1000 mg/kg Bacillus licheniformis).The temperature of the normal temperature group was 20-25℃,while that of the other two groups was artificially simulated the summer temperature.The plasma cortisol content was determined during the experiment.After the test,thyroid weight was measured and thyroid index was calculated.The changes of thyroid tissue under light microscope were observed,and changes of thyroid follicular epithelial cells and parathyroid eosinophils were observed under transmission electron microscope.The results showed that Bacillus licheniformis could significantly reduce cortisol content and increase thyroid weight and index in male rabbits exposed to heat stress for 15 d(P<0.01).Bacillus licheniformis significantly improved the structure of thyroid follicles,as evidenced by a clearer membrane structure,reduced nuclear atrophy and intracellular organelle damage,and it reduced mitochondrial vacuolation in parathyroid eosinophils.Therefore,adding 1000 mg/kg Bacillus licheniformis to the diet of male rabbits could effectively reduce the content of plasma cortisol and relieve heat damage to the thyroid and parathyroid glands in heat-stressed male rabbits.Bacillus licheniformis played an important role in ensuring the normal reproduction function of male rabbits.

关 键 词：地衣芽孢杆菌 热应激 公兔 甲状腺 甲状旁腺 

分 类 号：S852.2[农业科学—基础兽医学] S852.61[农业科学—兽医学]