敲低胎盘特异性蛋白8(PLAC8)抑制人胚胎干细胞增殖并促进其凋亡  

Knockdown of placental-specific protein 8(PLAC8)inhibits proliferation and promotes apoptosis of human embryonic stem cells

作　　者：李如梅 李敏[2] 陈蕾[1,2] LI Rumei;LI Min;CHEN Lei(Department of Obstetrics and Gynecology,Navy Clinical College,Anhui Medical University,Hefei 230032;Department of Obstetrics and Gynecology,Sixth Medical Center of PLA General Hospital,Beijing 100048,China)

机构地区：[1]安徽医科大学海军临床学院妇产科,安徽合肥230032 [2]解放军总医院第六医学中心妇产科,北京100048

出　　处：《细胞与分子免疫学杂志》2020年第12期1089-1094,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(8170060984);海军“十二五”项目(CHJ11J015)。

摘　　要：目的探讨胎盘特异性蛋白8(PLAC8)对人胚胎干细胞(hESC)增殖和凋亡的影响。方法实时荧光定量PCR和Western blot法检测空载体组、PLAC8短发夹RNA(shPLAC8)组、过表达PLAC8(PLAC8-OE)组感染hESC的效率;以及各组细胞增殖细胞核抗原(PCNA)和细胞周期蛋白D1(CCND1)的mRNA和蛋白水平。流式细胞术检测敲低和过表达PLAC8对hESC凋亡的影响。结果PLAC8敲低组的CCND1、PCNA的mRNA和蛋白水平降低,细胞凋亡增加;PLAC8过表达组CCND1和PCNA的mRNA和蛋白表达略上调,细胞凋亡略有下降,但差异均无统计学意义。结论敲低PLAC8的表达会抑制hESC增殖并促进其凋亡。Objective To investigate the effect of placenta-specific protein 8(PLAC8)on the proliferation and apoptosis of human embryonic stem cells(hESCs).Methods Real-time quantitative PCR and Western blotting were used to detect the infection efficiency of hESCs in short hairpin RNA negative control group,short hairpin RNA PLAC8(shPLAC8)group,and PLAC8 over-expression(PLAC8-OE)group.The mRNA and protein levels of proliferation cell nuclear antigen(PCNA)and cyclin D1(CCND1)in each group were also detected by the above methods.The flow cytometry was used to test the effect of PLAC8 knockdown or over-expression on the apoptosis of hESCs.Results The mRNA and protein levels of CCND1 and PCNA in the shPLAC8 group decreased,and cell apoptosis increased.The mRNA and protein expression of CCND1 and PCNA in the PLAC8 over-expression group were slightly up-regulated,while the apoptosis was slightly down-regulated,but the difference was not statistically significant.Conclusion The knockdown of PLAC8 expression can inhibit the proliferation and promote the apoptosis of hESCs.

关 键 词：人胚胎干细胞(hESC) 胎盘特异性蛋白8(PLAC8) 细胞增殖 细胞凋亡 

分 类 号：R392-33[医药卫生—免疫学] Q813.7[医药卫生—基础医学]