严重烫伤MODS小鼠的sTREM-1抗炎作用研究  

Study on the anti-inflammatory effect of sTREM-1 in severely scalded MODS mice

作　　者：骆沿极 刘安娜 王占科 肖创清[1] 胡亚芳 LUO Yanji;LIU Anna;WANG Zhanke;XIAO chuangqing;HU Yafang(Department of Clinical Laboratory,the Second Affiliated Hospital of Hunan Normal University/the 921th Hospital of Chinese People′s Liberation Army Joint Logistic Support Force,Changsha,Hunan 410000,China;Department of Clinical Laboratory,the 908th Hospital of Chinese People′s Liberation Army Joint Logistic Support Force,Nanchang,Jiangxi 330002,China)

机构地区：[1]湖南师范大学附属第二医院/中国人民解放军联勤保障部队第921医院检验科,湖南长沙410000 [2]中国人民解放军联勤保障部队第908医院检验科,江西南昌330002

出　　处：《国际检验医学杂志》2021年第5期531-535,共5页International Journal of Laboratory Medicine

基　　金：国家科技支撑计划子课题(2015BAI32H00)。

摘　　要：目的研究严重烫伤多器官功能障碍综合征(MODS)小鼠可溶性髓样细胞触发受体-1/细菌脂多糖(sTREM-1/LPS)比值的变化及sTREM-1的抗炎作用。方法200只无特定病原体(SPF)级的昆明小鼠按随机数字表法分为烫伤1 d组(30只)、烫伤3 d组(30只)、烫伤5 d组(30只)、髓样细胞触发受体-1(TREM-1)/Fc融合蛋白治疗1 d组(30只)、TREM-1/Fc融合蛋白治疗3 d组(30只)、TREM-1/Fc融合蛋白治疗5 d组(30只)和对照组(20只)。除对照组外,其余各组均为严重烫伤MODS小鼠。观察各组小鼠的sTREM-1/LPS比值、炎症因子肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平及各组小鼠的病死率。结果TREM-1/Fc融合蛋白治疗5 d组小鼠病死率小于烫伤5 d组,差异有统计学意义(P<0.05)。TREM-1/Fc融合蛋白治疗1 d组小鼠TNF-α和IL-6低于烫伤1 d组,差异有统计学意义(P<0.05);TREM-1/Fc融合蛋白治疗3 d组小鼠TNF-α和IL-6低于烫伤3 d组,差异有统计学意义(P<0.05);TREM-1/Fc融合蛋白治疗5 d组小鼠TNF-α和IL-6低于烫伤5 d组,差异有统计学意义(P<0.05)。烫伤1 d组、烫伤3 d组、烫伤5 d组和TREM-1/Fc融合蛋白治疗1 d组、TREM-1/Fc融合蛋白治疗3 d组、TREM-1/Fc融合蛋白治疗5 d组小鼠血浆sTREM-1/LPS比值与对照组比较,差异有统计学意义(P<0.05)。结论sTREM-1或TREM-1/Fc融合蛋白具有抗炎作用并能降低MODS的病死率。Objective To study the significance of soluble triggering receptor expressed on myeloid cell-1/lipopolysaccharide(sTREM-1/LPS ratio)and the anti-inflammatory effect of sTREM-1 in severely scalded multiple organ dysfunction syndrome(MODS)mice.Methods Two hundred Kunming mice of specific pathogen free(SPF)grade were randomly divided into seven groups:group of 1 d after scald(30 mice),group of 3 d after scald(30 mice),group of 5 d after scald(30 mice),group of 1 d after treatment with TREM-1/Fc fusion protein(30 mice),group of 3 d after treatment with TREM-1/Fc fusion protein(30 mice),group of 5 d after treatment with TREM-1/Fc fusion protein(30 mice),and the control group(20 mice).Except for the control group,the rest groups were severely scalded MODS mice.Observe the sTREM-1/LPS ratio,the levels of inflammatory cytokines(TNF-αand IL-6),and the mortality rate of mice in each group.Results The mortality rate of the group of 5 d after treatment with TREM-1/Fc fusion protein was lower than that of the group of 5 d after scald,the difference was statistically significant(P<0.05).TNF-αand IL-6 levels in the group of 1 d after treatment with TREM-1/Fc fusion protein were lower than those in group of 1 d after scald,the difference was statistically significant(P<0.05).TNF-αand IL-6 levels in the group of 3 d after treatment with TREM-1/Fc fusion protein were lower than those in group of 3 d after scald,the difference was statistically significant(P<0.05).TNF-αand IL-6 levels in the group of 5 d after treatment with TREM-1/Fc fusion protein were lower than those in group of 5 d after scald,the difference was statistically significant(P<0.05).The plasma sTREM-1/LPS ratios of groups of 1 d,3 d,5 d after scald and groups of 1 d,3 d,5 d after treatment with TREM-1/Fc fusion protein were statistically significant compared with that in control group(P<0.05).Conclusion sTREM-1 or TREM-1/Fc fusion protein has anti-inflammatory effects and can reduce MODS mortality rate.

关 键 词：可溶性髓样细胞触发受体-1 细菌脂多糖 炎症因子 多器官功能障碍综合征 

分 类 号：R459.7[医药卫生—急诊医学]